Detailed information of TA system TA022608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4190..4833
• Replicon: chromosome
• Accession: NC_010172
• Organism: Methylobacterium extorquens PA1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MEXT_RS00020
• Protein ID: WP_012251833.1
• Coordinates: 4190..4594 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MEXT_RS00025
• Protein ID: WP_012251834.1
• Coordinates: 4594..4833 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEXT_RS00005	44..1549	+	1506	WP_012251830.1	chromosomal replication initiator protein DnaA	-
MEXT_RS00010	1870..2991	+	1122	WP_012251831.1	DNA polymerase III subunit beta	-
MEXT_RS00015	3032..4186	+	1155	WP_012251832.1	DNA replication/repair protein RecF	-
MEXT_RS00020	4190..4594	-	405	WP_012251833.1	type II toxin-antitoxin system VapC family toxin	Toxin
MEXT_RS00025	4594..4833	-	240	WP_012251834.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MEXT_RS00030	4936..6783	+	1848	WP_012251835.1	DNA helicase RecQ	-
MEXT_RS00035	6841..7179	-	339	WP_012251836.1	hypothetical protein	-
MEXT_RS00040	7495..8181	+	687	WP_003598308.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-128)

Antitoxin

• Antitoxin VapB-like (1-75)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14308.34 Da
• Isoelectric Point: 5.1418

>T22608 WP_012251833.1 NC_010172:c4594-4190 [Methylorubrum extorquens PA1]
MFVDASAIIAIIAREPQAETLTARLEEAPVRYTSAVAVLETVMALRRLGAGSIAQAEQLVRHLLEHADIEEVPITTRESA
AAIAAYARYGKGQGHPAQLNLGDCFAYACARTRNVPLLFVGNDFSQTDIAAALA

Nucleotide

• Length: 405 bp

>T22608 NC_010172:c4594-4190 [Methylorubrum extorquens PA1]
ATGTTCGTAGACGCGTCGGCGATCATCGCGATCATCGCACGCGAGCCGCAGGCGGAAACATTGACGGCCCGGCTCGAGGA
GGCGCCCGTCCGCTATACCTCGGCAGTGGCCGTCTTGGAAACGGTGATGGCCTTGCGGCGCCTCGGTGCGGGATCGATCG
CGCAAGCAGAGCAACTGGTGCGACATCTGCTCGAGCATGCGGATATTGAGGAAGTCCCCATCACAACGCGGGAGTCGGCT
GCGGCCATCGCCGCATACGCGCGCTACGGCAAAGGCCAGGGCCACCCTGCCCAACTCAACCTCGGCGACTGCTTCGCCTA
TGCCTGCGCTCGAACGCGCAACGTCCCGCTTCTCTTCGTCGGCAACGACTTCTCGCAGACCGACATCGCCGCTGCATTGG
CCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8977.27 Da
• Isoelectric Point: 10.0862

>AT22608 WP_012251834.1 NC_010172:c4833-4594 [Methylorubrum extorquens PA1]
MPLNIRSEEVNRLAEKLASQARVSKTEAVRMALANELQRRESSLPLSERIKPIQDAFARWPKTGMKADKAFFDDMNGES

Nucleotide

• Length: 240 bp

>AT22608 NC_010172:c4833-4594 [Methylorubrum extorquens PA1]
ATGCCCTTGAACATCCGCAGCGAGGAGGTGAACCGCCTCGCCGAGAAGCTGGCATCGCAGGCTCGGGTGAGCAAGACCGA
GGCGGTGCGTATGGCGTTGGCCAACGAACTGCAACGCCGGGAATCGTCGCTGCCGCTGTCAGAGCGGATCAAGCCGATCC
AAGATGCCTTCGCGCGTTGGCCGAAGACGGGTATGAAAGCCGACAAGGCCTTCTTCGACGACATGAACGGCGAGTCCTGA