Detailed information of TA system TA226056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2664827..2665413
• Replicon: chromosome
• Accession: NZ_CP087696
• Organism: Xylella taiwanensis strain PLS16B

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Z9JFU4
• Locus tag: LPH57_RS11155
• Protein ID: WP_038273324.1
• Coordinates: 2665159..2665413 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Z9JGM5
• Locus tag: LPH57_RS11150
• Protein ID: WP_038273322.1
• Coordinates: 2664827..2665162 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH57_RS11125 (LPH57_11125)	2660255..2661463	+	1209	WP_038272829.1	hypothetical protein	-
LPH57_RS11130 (LPH57_11130)	2661965..2662285	-	321	WP_038272832.1	hypothetical protein	-
LPH57_RS11135 (LPH57_11135)	2662357..2663073	-	717	WP_160165226.1	portal protein	-
LPH57_RS11140 (LPH57_11140)	2663070..2664176	-	1107	WP_114867106.1	phage terminase large subunit	-
LPH57_RS11145 (LPH57_11145)	2664173..2664574	-	402	WP_114867107.1	lysozyme	-
LPH57_RS11150 (LPH57_11150)	2664827..2665162	-	336	WP_038273322.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LPH57_RS11155 (LPH57_11155)	2665159..2665413	-	255	WP_038273324.1	type II toxin-antitoxin system HicA family toxin	Toxin
LPH57_RS11160 (LPH57_11160)	2665515..2666213	-	699	WP_114867108.1	hypothetical protein	-
LPH57_RS11165 (LPH57_11165)	2666170..2667363	+	1194	WP_230428203.1	hypothetical protein	-
LPH57_RS11170 (LPH57_11170)	2667367..2667639	+	273	WP_114867070.1	VRR-NUC domain-containing protein	-
LPH57_RS11175 (LPH57_11175)	2667641..2667991	-	351	WP_114867109.1	DUF1640 domain-containing protein	-
LPH57_RS11180 (LPH57_11180)	2668067..2669413	+	1347	WP_114867110.1	DEAD/DEAH box helicase	-
LPH57_RS11185 (LPH57_11185)	2669428..2670312	+	885	WP_114867111.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2660255..2678268	18013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-80)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9561.95 Da
• Isoelectric Point: 11.1842

>T226056 WP_038273324.1 NZ_CP087696:c2665413-2665159 [Xylella taiwanensis]
MKRKHAKTLSTIDTRPVSANIQWRDIEALFLELGATVEEREGSRVLVRLFGERRVFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Nucleotide

• Length: 255 bp

>T226056 NZ_CP121766:c4227232-4226933 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12318.98 Da
• Isoelectric Point: 4.7313

>AT226056 WP_038273322.1 NZ_CP087696:c2665162-2664827 [Xylella taiwanensis]
MKNIMTIEGFKAIITYDPDIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACEEDDVEPRKSYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEIHI

Nucleotide

• Length: 336 bp

>AT226056 NZ_CP121766:c4226936-4226646 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: Z9JFU4

Antitoxin

• AlphaFold DB: Z9JGM5