Detailed information of TA system TA022604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 962887..963446
• Replicon: chromosome
• Accession: NC_010169
• Organism: Brucella suis ATCC 23445

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BSUIS_RS04695
• Protein ID: WP_004688321.1
• Coordinates: 963201..963446 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BSUIS_RS04690
• Protein ID: WP_002964097.1
• Coordinates: 962887..963201 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSUIS_RS04645	958577..958780	-	204	WP_004690812.1	hypothetical protein	-
BSUIS_RS04650	958783..958998	-	216	WP_002971459.1	hypothetical protein	-
BSUIS_RS04655	958995..959198	-	204	WP_002964088.1	hypothetical protein	-
BSUIS_RS04660	959248..959967	+	720	WP_006072696.1	hypothetical protein	-
BSUIS_RS04665	959964..960194	+	231	WP_002964091.1	hypothetical protein	-
BSUIS_RS04670	960191..960466	+	276	WP_002964092.1	hypothetical protein	-
BSUIS_RS04675	960489..961076	+	588	WP_004683738.1	hypothetical protein	-
BSUIS_RS04680	961311..962021	+	711	WP_004683739.1	porin family protein	-
BSUIS_RS04685	962369..962539	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BSUIS_RS04690	962887..963201	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BSUIS_RS04695	963201..963446	-	246	WP_004688321.1	BrnT family toxin	Toxin
BSUIS_RS04700	963579..964217	+	639	Protein_916	tRNA-guanine transglycosylase	-
BSUIS_RS17590	964287..965044	+	758	WP_144313581.1	IS5 family transposase	-
BSUIS_RS04715	965045..965818	+	774	WP_006072701.1	hypothetical protein	-
BSUIS_RS04720	965953..967599	+	1647	WP_006072703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	956793..970915	14122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T22604 WP_004688321.1 NC_010169:c963446-963201 [Brucella suis ATCC 23445]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T22604 NC_010169:c963446-963201 [Brucella suis ATCC 23445]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT22604 WP_002964097.1 NC_010169:c963201-962887 [Brucella suis ATCC 23445]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT22604 NC_010169:c963201-962887 [Brucella suis ATCC 23445]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA