Detailed information of TA system TA226027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 30817..31502
• Replicon: chromosome
• Accession: NZ_CP087692
• Organism: Xylella taiwanensis strain PLS427

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LPH51_RS00150
• Protein ID: WP_230439258.1
• Coordinates: 30817..31194 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LPH51_RS00155
• Protein ID: WP_038272460.1
• Coordinates: 31230..31502 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH51_RS00125 (LPH51_00125)	26171..27382	-	1212	WP_038272508.1	signal recognition particle-docking protein FtsY	-
LPH51_RS00130 (LPH51_00130)	28227..28700	+	474	WP_038272510.1	LEA type 2 family protein	-
LPH51_RS00135 (LPH51_00135)	28976..29590	+	615	WP_081755477.1	HNH endonuclease	-
LPH51_RS00140 (LPH51_00140)	30174..30419	+	246	WP_230428284.1	hypothetical protein	-
LPH51_RS00145 (LPH51_00145)	30410..30754	+	345	WP_038272464.1	DUF3653 domain-containing protein	-
LPH51_RS00150 (LPH51_00150)	30817..31194	-	378	WP_230439258.1	type II toxin-antitoxin system VapC family toxin	Toxin
LPH51_RS00155 (LPH51_00155)	31230..31502	-	273	WP_038272460.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LPH51_RS00160 (LPH51_00160)	31592..32719	-	1128	WP_114867127.1	zonular occludens toxin domain-containing protein	-
LPH51_RS00165 (LPH51_00165)	32732..33076	-	345	WP_038273069.1	DUF2523 family protein	-
LPH51_RS00170 (LPH51_00170)	33079..34464	-	1386	WP_230428213.1	furin-like repeat-containing protein	-
LPH51_RS00175 (LPH51_00175)	34644..34880	-	237	WP_230428212.1	major capsid protein	-
LPH51_RS00180 (LPH51_00180)	34889..35095	-	207	WP_038273075.1	hypothetical protein	-
LPH51_RS00185 (LPH51_00185)	35098..35412	-	315	WP_230428211.1	hypothetical protein	-
LPH51_RS00190 (LPH51_00190)	35688..36275	-	588	WP_038273076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	28928..58413	29485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (13-111)

Antitoxin

• SpoVT-AbrB domain (16-52)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13772.31 Da
• Isoelectric Point: 8.1155

>T226027 WP_230439258.1 NZ_CP087692:c31194-30817 [Xylella taiwanensis]
MIRNPFGLVACRIEKVDPKEVCTSIVVAAELRYGCAKKGSTKLLAKVKLMLETIPVLPLDIPTDAEYGRIRAELESVGQT
IGLNDLLIGTHAYVLGMTLVTDNVKEFKRIRGLKIENWLECASSV

Nucleotide

• Length: 378 bp

>T226027 NZ_CP121671:416878-417228 [Halobacillus naozhouensis]
GTGATAGTCAAACGAGGAGACGTTTATTTCGCTGATTTGTCCCCGGTTGTAGGATCAGAACAAGGCGGGGTACGCCCTGT
TCTCATCTTGCAAAATGATATTGGGAATCGTTTCAGCCCTACAGTTATTATCGCGGCAATAACCGCGCAGATTCAAAAAG
CTAAACTTCCTACACATGTTGAAATTGATGCTGAAAAATATGGTTTCGAACGCAATTCTGTTATTCTGCTGGAACAAATC
AGAACGATTGATAAACAACGTTTGACCGATAAAATCACACATCTGGATGGGCCGATGATGCAACAGGTTAACGAATCCTT
GCAAATCAGCCTTGGACTTATTGATTTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10331.95 Da
• Isoelectric Point: 4.5216

>AT226027 WP_038272460.1 NZ_CP087692:c31502-31230 [Xylella taiwanensis]
MSISMPSSRLKEAKLFRNNRSQAVRIPVEFELPGDRVLIHREGSKLIIEPVSMPTNIIELLAQWKTEDPLGLEDQFPEIE
DMLAKPDDIF

Nucleotide

• Length: 273 bp

>AT226027 NZ_CP121671:416591-416872 [Halobacillus naozhouensis]
ATGGTTTTGTCCGACAGTATGCAGGAGATTGTCATTCGTATTCCAGATAGCCTACTAAGTGAATTAGATGGTCATGTTCA
ACTCGAAAACAGTGATCGGAGCGATTTCATGTGTAAAGCAACAAAAATGTATTTGCGGGAAGAAAAGCAACGCCATATTA
GAGAATCCATGCGCCGTGGATATATGGAAATGGCTAAAATTAATTTAACTATCGCTTCAGAAGCGTTTCAGGCCGAAGAG
GAGGCAGATCACACCCTGGAGCAATTAGTGAGCGGGGTGTGA