Detailed information of TA system TA226014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2657393..2657979
• Replicon: chromosome
• Accession: NZ_CP087689
• Organism: Xylella taiwanensis strain PLS415

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Z9JFU4
• Locus tag: LPH50_RS11050
• Protein ID: WP_038273324.1
• Coordinates: 2657725..2657979 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Z9JGM5
• Locus tag: LPH50_RS11045
• Protein ID: WP_038273322.1
• Coordinates: 2657393..2657728 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH50_RS11020 (LPH50_11020)	2652821..2654029	+	1209	WP_038272829.1	hypothetical protein	-
LPH50_RS11025 (LPH50_11025)	2654531..2654851	-	321	WP_038272832.1	hypothetical protein	-
LPH50_RS11030 (LPH50_11030)	2654923..2655639	-	717	WP_160165226.1	portal protein	-
LPH50_RS11035 (LPH50_11035)	2655636..2656718	-	1083	WP_051482382.1	phage terminase large subunit	-
LPH50_RS11040 (LPH50_11040)	2656739..2657140	-	402	WP_114867107.1	lysozyme	-
LPH50_RS11045 (LPH50_11045)	2657393..2657728	-	336	WP_038273322.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LPH50_RS11050 (LPH50_11050)	2657725..2657979	-	255	WP_038273324.1	type II toxin-antitoxin system HicA family toxin	Toxin
LPH50_RS11055 (LPH50_11055)	2658081..2658779	-	699	WP_114867108.1	hypothetical protein	-
LPH50_RS11060 (LPH50_11060)	2658736..2659929	+	1194	WP_230428203.1	hypothetical protein	-
LPH50_RS11065 (LPH50_11065)	2659933..2660205	+	273	WP_114867070.1	VRR-NUC domain-containing protein	-
LPH50_RS11070 (LPH50_11070)	2660207..2660557	-	351	WP_114867109.1	DUF1640 domain-containing protein	-
LPH50_RS11075 (LPH50_11075)	2660633..2661979	+	1347	WP_114867110.1	DEAD/DEAH box helicase	-
LPH50_RS11080 (LPH50_11080)	2661994..2662878	+	885	WP_114867111.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2652821..2671904	19083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-80)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9561.95 Da
• Isoelectric Point: 11.1842

>T226014 WP_038273324.1 NZ_CP087689:c2657979-2657725 [Xylella taiwanensis]
MKRKHAKTLSTIDTRPVSANIQWRDIEALFLELGATVEEREGSRVLVRLFGERRVFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Nucleotide

• Length: 255 bp

>T226014 NZ_CP121662:1577407-1577847 [Rhizobium sp. CB3090]
GTGACTTTCCTGCTTGATGTCAATGTTCTGATCGCCTTGTTCGATCCGGCGCACGTCTCTCATGATATTGCGCATAACTG
GTTTAGCTCGACCGGCAATCAAAGCTGGGCGACATGTCCGTTGACAGAAAACGGCGTCATTCGAATTCTCGGCCAATCCT
CCTACCCCAATTCCCCCGGTCCGCCGTCGACGGTTGTACCTTTGATTGAAGAACTTCGACGCTTGCCGGGTCACGAGTTT
TGGGCCGATGACATAAGTTTGACGGATGCGAGTCTGATCGATCCTGCGCGACTTCTGACGCCCGGACAAGTCACGGACAG
CTATCTATTGGCTCTTGCGAAATTCCGCCGTGGCAAGCTTGCCACTCTTGATCGCCGGTTGTCGACAGCGGCAGTGAAAG
ACGGGCGTGCCGCGCTTCATATCATCGGCAGCGCCACCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12318.98 Da
• Isoelectric Point: 4.7313

>AT226014 WP_038273322.1 NZ_CP087689:c2657728-2657393 [Xylella taiwanensis]
MKNIMTIEGFKAIITYDPDIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACEEDDVEPRKSYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEIHI

Nucleotide

• Length: 336 bp

>AT226014 NZ_CP121662:1577183-1577410 [Rhizobium sp. CB3090]
ATGCGAACGACCTTGGCGATCGACGATGACGTGCTTGCTGCAGCGAAAGCTATGGCGGAACAACAGCGCCGCAGCGTTGG
CGATGTAATTTCAGATTTGGCCCGCCGCTCTCTCAGCCGCCCTCAGATGGGAGCGGAACGAAACGGCATTCCTCTATTGG
TCCCGAAATCAAGCACCCCTCCCATCACGCTCGAAATCGTCAATAAACTTCGTGATGAACTCCCGTGA

Structures

Toxin

• AlphaFold DB: Z9JFU4

Antitoxin

• AlphaFold DB: Z9JGM5