Detailed information of TA system TA226006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 38942..39483
• Replicon: chromosome
• Accession: NZ_CP087688
• Organism: Xylella taiwanensis strain PLS413

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Z9JGT1
• Locus tag: LPH64_RS00225
• Protein ID: WP_038273166.1
• Coordinates: 39160..39483 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Z9JGL9
• Locus tag: LPH64_RS00220
• Protein ID: WP_038273164.1
• Coordinates: 38942..39163 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH64_RS00170 (LPH64_00170)	34518..34754	-	237	WP_230428212.1	major capsid protein	-
LPH64_RS00175 (LPH64_00175)	34763..34969	-	207	WP_038273075.1	hypothetical protein	-
LPH64_RS00180 (LPH64_00180)	34972..35286	-	315	WP_230428211.1	hypothetical protein	-
LPH64_RS00185 (LPH64_00185)	35562..36149	-	588	WP_038273076.1	hypothetical protein	-
LPH64_RS00190 (LPH64_00190)	36346..36489	-	144	WP_160165233.1	hypothetical protein	-
LPH64_RS00195 (LPH64_00195)	36609..36809	-	201	WP_038273077.1	hypothetical protein	-
LPH64_RS00200 (LPH64_00200)	36858..37121	-	264	WP_162814095.1	hypothetical protein	-
LPH64_RS00205 (LPH64_00205)	37125..37286	-	162	WP_160165236.1	hypothetical protein	-
LPH64_RS00210 (LPH64_00210)	37576..37905	+	330	WP_230428286.1	DUF3693 domain-containing protein	-
LPH64_RS00215 (LPH64_00215)	37994..38494	-	501	Protein_42	tyrosine-type recombinase/integrase	-
LPH64_RS00220 (LPH64_00220)	38942..39163	+	222	WP_038273164.1	antitoxin MazE family protein	Antitoxin
LPH64_RS00225 (LPH64_00225)	39160..39483	+	324	WP_038273166.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LPH64_RS00230 (LPH64_00230)	39584..40732	-	1149	WP_069636227.1	zonular occludens toxin domain-containing protein	-
LPH64_RS00235 (LPH64_00235)	40732..41073	-	342	WP_038273089.1	DUF2523 family protein	-
LPH64_RS00240 (LPH64_00240)	41077..42144	-	1068	WP_152536670.1	virulence factor TspB C-terminal domain-related protein	-
LPH64_RS00245 (LPH64_00245)	42612..42830	-	219	WP_051482398.1	major capsid protein	-
LPH64_RS00250 (LPH64_00250)	42839..43048	-	210	WP_230428208.1	hypothetical protein	-
LPH64_RS00255 (LPH64_00255)	43048..43341	-	294	WP_038273093.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	30465..49342	18877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (9-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11807.97 Da
• Isoelectric Point: 9.1188

>T226006 WP_038273166.1 NZ_CP087688:39160-39483 [Xylella taiwanensis]
MTQRGDLVTVSLQGDYGKPRPALILQSDLLMELDSVVLCPVTSELRNAIFRITVEPNIANGLRTLSQIMVDKISTLPRSK
ISKPFGHLNDERIKAVEKALLLIVGII

Nucleotide

• Length: 324 bp

>T226006 NZ_CP121656:c26899-25982 [Rhizobium sp. CB3171]
ATGACTAAAATCATCAAGGACAAATCGGCGTCTATCCGCGCAAGACTGCTCATGCACGCAAAGCAGTCTGGTGAGGAATA
TCAGCGTACACTAACGCGCTTCGCCATTGAACGTTTGTTGTTTCGCCTCAGTCAATCGGGCGTGCGCGACCGCTACGTTC
TCAAAGGAGCGATGCTCTTCGTAACCTGGCCAGAGAATGTCTTTCGACCCACCGGCGATCTCGATCTGTTGGGTCACGGC
GATCCTGACCCCGCTGCGATAGTTGAAACCTTCCAGCAAATCTGTGCGGTCGAAGCCGACGACGGTTTGCACTTCGACCT
CGCGAGCATCAAGGTCGATCCGGTCCGGGAAGCGGACAAGTATCAGGGCGCTCGTGTCATGCTGAATGGCGAGCTCGCCA
AGGCGATCATTCGAGTGCAAGTTGACATCGGCTTCGGAGATCATGTCTATCCGGCGCCTAAGCTGGCGTCGTTCCCGAGC
ATTCTTCCGGATCTGCCGACGGCCAGCATACTGATGTATCCGCCCGAGACCGTCGTCGCGGAAAAGTTCGAAGCAATGAT
CCGTTTCGGACTGGCGAACGGCCGGATCAAGGATTTCTATGACATCTGGCTCACTACACAGATTTTTCAATTTCAGCTCT
CGACTGTGATCGATGCGGTCGCCGGCACATTGGCGCGCCGCGGAACGGCCGTCCCAACCGCGAGGCCCGAGGGATTTTCG
CAGGCCTACGCTACCATCGCTGCGGAGAAAGGTTTGTGGAGCGGTTTTCTCGAGCGCAACCCGCCAATGCTGGCACCTCC
CGCCTTTCCTGAGCTTCAGCGCCAGCTCACGCGTTTCTTCGAGCCAGTCATTGCGGGCCTCAATGCCCCCGGCGGGGCCG
AGAGCCGCTGGGACCCAGTCGAATACAGCTGGCGATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8490.65 Da
• Isoelectric Point: 8.0827

>AT226006 WP_038273164.1 NZ_CP087688:38942-39163 [Xylella taiwanensis]
MAFIHEPTSKVARHRKQMRAAGLRPVQFWVPDTRTPEFAAEIRSQCRSLKNDPEEADALRFTEEAITHIEGWK

Nucleotide

• Length: 222 bp

>AT226006 NZ_CP121656:c27498-26896 [Rhizobium sp. CB3171]
ATGAATTATCGGGATAGCGCGCTCGAACTGGCCAGATCTCGCGGCATCGTGCGGGCCCGTGATTTCAAGTCGGCCGGCAT
CCCCCTCGTCTATCTGAAACGCCTGACCGATTCCGGCGACCTCGTGCGACTTGGACGTGGACTGTATCAGGATCCAGACC
GTGTTGGTGAGCACGTCGCGCACGATCTGGCAGAAGCTACCCGGTTGATGGCGAACGGCACAGTCTGCCTGATCAGCGCA
CTTCGCCAACACGAATTGACCACTCAGCTACCGCATGCGGTCTGGATGATGATCCCTCATAAGGCAAGAGCGCCGATATC
ACCATCGTTTCCAGTAAAAATCGTGCGGGCAACCGGTGATGCCCTAACATCCGGCATTGAGAGGATTTCGGTCGAAGGCG
TCGATGTGCCTATCTATGGCGTGGCAAAAACCGTAGCGGACTGCTTCAAATATCGGCGCCTTGTTGGCGAGGACGTCGCG
ATTGAAGCGCTTCGCGATGCCCTGCGATTTCGCAAGACGACACCGAACGAACTCATGAAATACGCAGCGATCGACCGGTG
CTCGGCGCGCATGGAGCCTCTGATCCGGGCGCTACAGGAATGA

Structures

Toxin

• AlphaFold DB: Z9JGT1

Antitoxin

• AlphaFold DB: Z9JGL9