Detailed information of TA system TA022600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-DUF971
• Location: 1158832..1159408
• Replicon: chromosome
• Accession: NC_010161
• Organism: Bartonella tribocorum CIP 105476

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A9ITS8
• Locus tag: BTR_RS05170
• Protein ID: WP_012231675.1
• Coordinates: 1159097..1159408 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A9ITS5
• Locus tag: BTR_RS05165
• Protein ID: WP_012231674.1
• Coordinates: 1158832..1159116 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTR_RS05130	1154064..1155551	-	1488	WP_012231669.1	DUF3987 domain-containing protein	-
BTR_RS13130	1155523..1155672	-	150	WP_169309787.1	hypothetical protein	-
BTR_RS12615	1155809..1155880	-	72	Protein_962	virulence-associated protein E	-
BTR_RS05140	1155979..1156179	-	201	WP_007347430.1	DNA-binding protein	-
BTR_RS05145	1156388..1157554	-	1167	WP_012231670.1	site-specific integrase	-
BTR_RS05150	1157717..1158007	+	291	Protein_965	DUF4043 family protein	-
BTR_RS05155	1158027..1158407	+	381	WP_012231672.1	hypothetical protein	-
BTR_RS05160	1158419..1158799	+	381	WP_012231673.1	hypothetical protein	-
BTR_RS05165	1158832..1159116	-	285	WP_012231674.1	putative addiction module antidote protein	Antitoxin
BTR_RS05170	1159097..1159408	-	312	WP_012231675.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTR_RS05175	1159482..1160189	+	708	WP_012231676.1	hypothetical protein	-
BTR_RS05180	1160191..1161648	+	1458	WP_012231677.1	hypothetical protein	-
BTR_RS05185	1161648..1162073	+	426	WP_012231678.1	hypothetical protein	-
BTR_RS05190	1162075..1162998	+	924	WP_012231679.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1108117..1191683	83566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-97)

Antitoxin

• Uncharacterized protein HI1420 (9-91)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11750.63 Da
• Isoelectric Point: 9.5068

>T22600 WP_012231675.1 NC_010161:c1159408-1159097 [Bartonella tribocorum CIP 105476]
MVAIIKTTDEFDEWLEKLKDRKAANIILTRLHRIRSGLLGDAKFFNGIGELRIHYGAGYRIYFTQKGDRIIILLCGGDKS
TQSNDIQKALSLVKDLKNEIENL

Nucleotide

• Length: 312 bp

>T22600 NC_010161:c1159408-1159097 [Bartonella tribocorum CIP 105476]
ATGGTAGCGATAATAAAAACAACAGATGAATTCGATGAATGGTTAGAAAAGCTTAAAGACAGAAAAGCTGCTAATATAAT
TCTTACAAGACTTCATAGAATTCGTTCAGGACTTTTGGGTGATGCGAAGTTCTTTAATGGCATAGGTGAATTACGTATCC
ATTATGGGGCTGGTTACAGAATTTATTTCACACAAAAAGGCGATAGAATCATTATATTACTTTGTGGTGGTGATAAATCC
ACACAATCCAATGATATTCAAAAAGCCTTATCTCTTGTAAAGGATCTTAAAAATGAAATTGAAAACCTTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10225.68 Da
• Isoelectric Point: 9.1009

>AT22600 WP_012231674.1 NC_010161:c1159116-1158832 [Bartonella tribocorum CIP 105476]
MKLKTFNFEKHLTTPESQEVFLNEAFKTGDAAHIADAIGIVARAQNMCTLAKETNRERSGLYRSLSKTGDPKLSTLVAVL
SALNLQLSVQSATS

Nucleotide

• Length: 285 bp

>AT22600 NC_010161:c1159116-1158832 [Bartonella tribocorum CIP 105476]
ATGAAATTGAAAACCTTTAACTTTGAAAAACACCTCACAACACCAGAATCTCAAGAAGTCTTTTTAAATGAAGCCTTCAA
AACCGGTGATGCTGCTCATATAGCGGATGCTATTGGTATCGTTGCTCGCGCTCAAAATATGTGCACCTTAGCAAAAGAAA
CCAATCGCGAACGTAGTGGATTATATCGTTCTTTAAGCAAAACGGGTGACCCTAAGCTTTCTACCTTAGTCGCTGTCTTA
TCTGCCCTTAATTTACAACTCTCGGTACAATCTGCTACTTCATAA

Structures

Toxin

• AlphaFold DB: A9ITS8

Antitoxin

• AlphaFold DB: A9ITS5