Detailed information of TA system TA022596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 546908..547461
• Replicon: chromosome
• Accession: NC_010161
• Organism: Bartonella tribocorum CIP 105476

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A9IPT5
• Locus tag: BTR_RS02480
• Protein ID: WP_012231069.1
• Coordinates: 547213..547461 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A9IPT2
• Locus tag: BTR_RS02475
• Protein ID: WP_012231068.1
• Coordinates: 546908..547216 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTR_RS02435	542434..543540	-	1107	WP_012230791.1	hypothetical protein	-
BTR_RS02440	543540..544367	-	828	WP_012230798.1	baseplate J/gp47 family protein	-
BTR_RS02445	544364..544705	-	342	WP_038473244.1	GPW/gp25 family protein	-
BTR_RS02450	544702..545367	-	666	WP_012231064.1	phage baseplate assembly protein V	-
BTR_RS02455	545348..545890	-	543	WP_012230801.1	hypothetical protein	-
BTR_RS02460	545940..546212	+	273	WP_012231065.1	toxin HicA	-
BTR_RS02465	546202..546519	+	318	WP_012231066.1	type II toxin-antitoxin system HicB family antitoxin	-
BTR_RS02470	546565..546837	+	273	WP_012231067.1	BrnA antitoxin family protein	-
BTR_RS02475	546908..547216	-	309	WP_012231068.1	BrnA antitoxin family protein	Antitoxin
BTR_RS02480	547213..547461	-	249	WP_012231069.1	BrnT family toxin	Toxin
BTR_RS02485	547556..547912	-	357	WP_012231070.1	hypothetical protein	-
BTR_RS02490	547914..548990	-	1077	WP_012231071.1	major capsid protein	-
BTR_RS02495	549003..549371	-	369	WP_012230814.1	head decoration protein	-
BTR_RS02500	549368..549712	-	345	WP_038474435.1	hypothetical protein	-
BTR_RS02505	549631..550698	-	1068	WP_038473315.1	S49 family peptidase	-
BTR_RS02510	550688..552259	-	1572	WP_012230821.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	476132..594109	117977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-100)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9351.99 Da
• Isoelectric Point: 8.6593

>T22596 WP_012231069.1 NC_010161:c547461-547213 [Bartonella tribocorum CIP 105476]
MIITYDEIKRHKNIEKHGLDFADLQIEFFETAIILPAKQGRFMAINVFTNNVIAVVFATLGDEAVSIISMRRASKKERSL
IL

Nucleotide

• Length: 249 bp

>T22596 NC_010161:c547461-547213 [Bartonella tribocorum CIP 105476]
ATGATTATTACTTATGATGAAATTAAACGACACAAAAACATTGAAAAACATGGGCTTGATTTTGCTGATCTTCAAATAGA
ATTTTTTGAAACAGCGATCATCCTTCCAGCAAAGCAAGGACGTTTTATGGCTATTAATGTTTTTACCAATAATGTTATTG
CAGTCGTTTTTGCTACACTTGGCGATGAAGCGGTTTCTATCATTTCTATGAGACGTGCGAGCAAAAAAGAAAGGAGTTTA
ATATTATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11524.11 Da
• Isoelectric Point: 4.9534

>AT22596 WP_012231068.1 NC_010161:c547216-546908 [Bartonella tribocorum CIP 105476]
MTKYPYIKPLTDEEEAEIQKEIAYDPDAPEATDEQIAQAKSFAEVFPDLAESIKRSRGRPQIDNPKEQVSIRLSPDVLKK
LKALGRGWQSKVDDILRKAVGL

Nucleotide

• Length: 309 bp

>AT22596 NC_010161:c547216-546908 [Bartonella tribocorum CIP 105476]
ATGACGAAATATCCATATATTAAACCTCTCACCGATGAAGAAGAGGCTGAAATTCAAAAAGAAATTGCATATGATCCAGA
CGCTCCAGAAGCGACAGATGAACAAATTGCTCAAGCAAAATCATTTGCAGAAGTATTTCCTGATTTAGCTGAAAGCATTA
AACGTTCTCGAGGGCGACCACAAATTGATAACCCTAAAGAGCAAGTATCAATTCGTTTGTCACCAGATGTTTTAAAAAAA
CTTAAAGCCTTGGGTCGTGGGTGGCAATCAAAAGTCGATGACATTTTACGTAAAGCAGTTGGGTTATAA

Structures

Toxin

• AlphaFold DB: A9IPT5

Antitoxin

• AlphaFold DB: A9IPT2