Detailed information of TA system TA225946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4730837..4732094
• Replicon: chromosome
• Accession: NZ_CP087674
• Organism: Pseudomonas aeruginosa strain P4970C

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LO758_RS22275
• Protein ID: WP_003449003.1
• Coordinates: 4730837..4731592 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LO758_RS22280
• Protein ID: WP_003086329.1
• Coordinates: 4731726..4732094 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO758_RS22265 (LO758_22265)	4726010..4728553	+	2544	WP_033876574.1	acyl-homoserine-lactone acylase QuiP	-
LO758_RS22270 (LO758_22270)	4728708..4730069	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
LO758_RS22275 (LO758_22275)	4730837..4731592	-	756	WP_003449003.1	RES family NAD+ phosphorylase	Toxin
LO758_RS22280 (LO758_22280)	4731726..4732094	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LO758_RS22285 (LO758_22285)	4732167..4733453	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
LO758_RS22290 (LO758_22290)	4733509..4735098	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
LO758_RS22295 (LO758_22295)	4735177..4735653	+	477	WP_003112531.1	hypothetical protein	-
LO758_RS22300 (LO758_22300)	4735656..4735931	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28292.45 Da
• Isoelectric Point: 7.7294

>T225946 WP_003449003.1 NZ_CP087674:c4731592-4730837 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T225946 NZ_CP121609:c2946073-2945684 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT225946 WP_003086329.1 NZ_CP087674:c4732094-4731726 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT225946 NZ_CP121609:c2946337-2946080 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7