Detailed information of TA system TA225938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2074082..2074739
• Replicon: chromosome
• Accession: NZ_CP087673
• Organism: Pseudomonas aeruginosa strain P96131

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8G3IXE4
• Locus tag: LO755_RS09815
• Protein ID: WP_003098540.1
• Coordinates: 2074557..2074739 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A6A9KT86
• Locus tag: LO755_RS09810
• Protein ID: WP_034014229.1
• Coordinates: 2074082..2074510 (-)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO755_RS09780 (LO755_09780)	2069165..2069482	+	318	WP_033977464.1	hypothetical protein	-
LO755_RS09785 (LO755_09785)	2069479..2069904	+	426	WP_033977466.1	hypothetical protein	-
LO755_RS09790 (LO755_09790)	2069901..2071019	+	1119	WP_033977468.1	DUF1376 domain-containing protein	-
LO755_RS09795 (LO755_09795)	2071013..2072824	+	1812	WP_023093240.1	DnaB-like helicase C-terminal domain-containing protein	-
LO755_RS09800 (LO755_09800)	2072821..2073105	+	285	WP_230444911.1	hypothetical protein	-
LO755_RS09805 (LO755_09805)	2073102..2073659	+	558	WP_003085729.1	hypothetical protein	-
LO755_RS09810 (LO755_09810)	2074082..2074510	-	429	WP_034014229.1	transcriptional regulator	Antitoxin
LO755_RS09815 (LO755_09815)	2074557..2074739	-	183	WP_003098540.1	type II toxin-antitoxin system HicA family toxin	Toxin
LO755_RS09820 (LO755_09820)	2074939..2075271	+	333	WP_003129668.1	phage holin, lambda family	-
LO755_RS09825 (LO755_09825)	2075268..2075885	+	618	WP_230444912.1	glycoside hydrolase family 19 protein	-
LO755_RS09830 (LO755_09830)	2075918..2076355	+	438	WP_230444913.1	lysis system i-spanin subunit Rz	-
LO755_RS09835 (LO755_09835)	2076352..2077095	+	744	WP_073673209.1	hypothetical protein	-
LO755_RS09840 (LO755_09840)	2077227..2077754	+	528	WP_023117090.1	terminase small subunit	-
LO755_RS09845 (LO755_09845)	2077726..2079681	+	1956	WP_229038204.1	phage terminase large subunit family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimV	2054019..2097968	43949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-78)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6746.86 Da
• Isoelectric Point: 11.0775

>T225938 WP_003098540.1 NZ_CP087673:c2074739-2074557 [Pseudomonas aeruginosa]
MKFSEFRRWLKAQGVTFEAGKGSHFKITAPNGKQTTFADHGAKEMPEPTRKAIIKQLGLK

Nucleotide

• Length: 183 bp

>T225938 NZ_CP121595:c3265401-3265012 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15758.92 Da
• Isoelectric Point: 4.6562

>AT225938 WP_034014229.1 NZ_CP087673:c2074510-2074082 [Pseudomonas aeruginosa]
MYDYAIRFEQDDSTPGVAVFCRDLPELNSYGDDKAHAIGEAVDAIESTLSLYVDQRREIPAASQAQPGERVIHLPAVTVA
KIALWNEMVRRDMRKADLCRLLGIAQTQGDRLVDFLHNTKMEAMENALSALGLRLSVNIEAA

Nucleotide

• Length: 429 bp

>AT225938 NZ_CP121595:c3265665-3265408 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6A9KT86