Detailed information of TA system TA225936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 147487..147992
• Replicon: chromosome
• Accession: NZ_CP087673
• Organism: Pseudomonas aeruginosa strain P96131

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: LO755_RS00660
• Protein ID: WP_003083773.1
• Coordinates: 147487..147768 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LO755_RS00665
• Protein ID: WP_003083775.1
• Coordinates: 147765..147992 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO755_RS00635 (LO755_00635)	142738..144087	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
LO755_RS00640 (LO755_00640)	144136..144822	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LO755_RS00645 (LO755_00645)	144923..145657	+	735	WP_003083764.1	GntR family transcriptional regulator	-
LO755_RS00650 (LO755_00650)	145837..146247	+	411	WP_003110659.1	aegerolysin family protein	-
LO755_RS00655 (LO755_00655)	146279..147187	-	909	WP_003083769.1	LysR family transcriptional regulator	-
LO755_RS00660 (LO755_00660)	147487..147768	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
LO755_RS00665 (LO755_00665)	147765..147992	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LO755_RS00670 (LO755_00670)	148168..148788	-	621	WP_003101226.1	hypothetical protein	-
LO755_RS00675 (LO755_00675)	148889..149389	+	501	WP_003083778.1	LEA type 2 family protein	-
LO755_RS00680 (LO755_00680)	149462..149803	+	342	WP_014603467.1	zinc ribbon domain-containing protein YjdM	-
LO755_RS00685 (LO755_00685)	149885..151312	-	1428	WP_003083784.1	GABA permease	-
LO755_RS00690 (LO755_00690)	151481..152974	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T225936 WP_003083773.1 NZ_CP087673:c147768-147487 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T225936 NZ_CP121590:7917-8204 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT225936 WP_003083775.1 NZ_CP087673:c147992-147765 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT225936 NZ_CP121590:8191-8505 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9