Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 26565..27090 Replicon plasmid P3-MCR-8
Accession NZ_CP087672
Organism Klebsiella pneumoniae strain KP53

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag LOM31_RS28675 Protein ID WP_013023785.1
Coordinates 26785..27090 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag LOM31_RS28670 Protein ID WP_001568025.1
Coordinates 26565..26783 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOM31_RS28650 21812..22780 + 969 WP_075211999.1 IS5 family transposase -
LOM31_RS28655 23252..24043 - 792 WP_221928726.1 ribbon-helix-helix domain-containing protein -
LOM31_RS28660 24226..25254 - 1029 WP_032445668.1 Abi family protein -
LOM31_RS28665 25415..25996 - 582 WP_072310991.1 hypothetical protein -
LOM31_RS28670 26565..26783 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LOM31_RS28675 26785..27090 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
LOM31_RS28680 27259..27654 + 396 WP_017899885.1 hypothetical protein -
LOM31_RS28685 27681..28004 + 324 WP_004197641.1 hypothetical protein -
LOM31_RS28690 28001..29017 + 1017 WP_118842534.1 hypothetical protein -
LOM31_RS28695 29215..30000 + 786 WP_046664219.1 site-specific integrase -
LOM31_RS28700 30449..31204 + 756 WP_001568031.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-8 / floR / tet(A) - 1..71532 71532
- flank IS/Tn mcr-8 - 15755..22780 7025


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T225935 WP_013023785.1 NZ_CP087672:26785-27090 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T225935 NZ_CP121589:6553-6912 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT225935 WP_001568025.1 NZ_CP087672:26565-26783 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT225935 NZ_CP121589:6912-7211 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References