Detailed information of TA system TA225932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 36215..37125
• Replicon: plasmid P1-tmexCD
• Accession: NZ_CP087670
• Organism: Klebsiella pneumoniae strain KP53

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: LOM31_RS26375
• Protein ID: WP_004181896.1
• Coordinates: 36655..37125 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: LOM31_RS26370
• Protein ID: WP_004181895.1
• Coordinates: 36215..36658 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOM31_RS26345	31371..31586	-	216	Protein_44	IS200/IS605 family transposase	-
LOM31_RS26350	31588..32157	+	570	Protein_45	transposase	-
LOM31_RS26355	32848..34335	+	1488	WP_023332914.1	group II intron reverse transcriptase/maturase	-
LOM31_RS26360	34421..34843	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LOM31_RS26365	34843..36114	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
LOM31_RS26370	36215..36658	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
LOM31_RS26375	36655..37125	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
LOM31_RS26380	37235..37495	-	261	WP_004026352.1	hypothetical protein	-
LOM31_RS26385	38194..39323	+	1130	Protein_52	IS3-like element ISKpn8 family transposase	-
LOM31_RS26390	39355..40323	+	969	WP_016151349.1	IS5 family transposase	-
LOM31_RS26395	40381..40629	+	249	Protein_54	IS3 family transposase	-
LOM31_RS26400	40648..41760	-	1113	WP_014386573.1	IS481-like element ISKpn28 family transposase	-
LOM31_RS26405	41832..41990	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4	-	1..280953	280953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T225932 WP_004181896.1 NZ_CP087670:36655-37125 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T225932 NZ_CP121587:3563-3850 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT225932 WP_004181895.1 NZ_CP087670:36215-36658 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT225932 NZ_CP121587:3837-4151 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9