Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5245196..5245821 Replicon chromosome
Accession NZ_CP087669
Organism Klebsiella pneumoniae strain KP53

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag LOM31_RS25760 Protein ID WP_004187928.1
Coordinates 5245196..5245579 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LOM31_RS25765 Protein ID WP_004150355.1
Coordinates 5245579..5245821 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOM31_RS25745 (5242562) 5242562..5243464 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LOM31_RS25750 (5243461) 5243461..5244096 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LOM31_RS25755 (5244093) 5244093..5245022 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LOM31_RS25760 (5245196) 5245196..5245579 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
LOM31_RS25765 (5245579) 5245579..5245821 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LOM31_RS25770 (5246015) 5246015..5246932 + 918 WP_004187929.1 alpha/beta hydrolase -
LOM31_RS25775 (5246946) 5246946..5247887 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LOM31_RS25780 (5247932) 5247932..5248369 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LOM31_RS25785 (5248366) 5248366..5249226 - 861 WP_004146232.1 virulence factor BrkB family protein -
LOM31_RS25790 (5249220) 5249220..5249819 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T225931 WP_004187928.1 NZ_CP087669:c5245579-5245196 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T225931 NZ_CP121586:1893255-1893437 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT225931 WP_004150355.1 NZ_CP087669:c5245821-5245579 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT225931 NZ_CP121586:1893530-1893934 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References