Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4642329..4643139 Replicon chromosome
Accession NZ_CP087669
Organism Klebsiella pneumoniae strain KP53

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag LOM31_RS22895 Protein ID WP_023279404.1
Coordinates 4642329..4642862 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LOM31_RS22900 Protein ID WP_002887278.1
Coordinates 4642873..4643139 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOM31_RS22890 (4641160) 4641160..4642281 + 1122 WP_002887282.1 cupin domain-containing protein -
LOM31_RS22895 (4642329) 4642329..4642862 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
LOM31_RS22900 (4642873) 4642873..4643139 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LOM31_RS22905 (4643242) 4643242..4644675 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
LOM31_RS22910 (4644665) 4644665..4645348 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LOM31_RS22915 (4645520) 4645520..4646905 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
LOM31_RS22920 (4646923) 4646923..4647267 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T225927 WP_023279404.1 NZ_CP087669:c4642862-4642329 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T225927 NZ_CP121583:3911714-3912103 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT225927 WP_002887278.1 NZ_CP087669:c4643139-4642873 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT225927 NZ_CP121583:3911450-3911707 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References