Detailed information of TA system TA225925

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3904344..3905116
• Replicon: chromosome
• Accession: NZ_CP087669
• Organism: Klebsiella pneumoniae strain KP53

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2I8S4E2
• Locus tag: LOM31_RS19285
• Protein ID: WP_065805889.1
• Coordinates: 3904344..3904733 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LOM31_RS19290
• Protein ID: WP_175364756.1
• Coordinates: 3904790..3905116 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOM31_RS19250 (3899644)	3899644..3900948	-	1305	WP_004147414.1	citrate synthase	-
LOM31_RS19255 (3900994)	3900994..3901134	-	141	WP_020804400.1	hypothetical protein	-
LOM31_RS19260 (3901459)	3901459..3901629	+	171	WP_002892370.1	hypothetical protein	-
LOM31_RS19265 (3901708)	3901708..3902109	-	402	WP_002892366.1	hydroxyisourate hydrolase	-
LOM31_RS19270 (3902505)	3902505..3902798	-	294	WP_002892360.1	hypothetical protein	-
LOM31_RS19275 (3903076)	3903076..3903241	+	166	Protein_3781	hypothetical protein	-
LOM31_RS19280 (3903412)	3903412..3904257	-	846	WP_065805890.1	DUF4942 domain-containing protein	-
LOM31_RS19285 (3904344)	3904344..3904733	-	390	WP_065805889.1	TA system toxin CbtA family protein	Toxin
LOM31_RS19290 (3904790)	3904790..3905116	-	327	WP_175364756.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LOM31_RS19295 (3905174)	3905174..3905395	-	222	WP_065805887.1	DUF987 domain-containing protein	-
LOM31_RS19300 (3905409)	3905409..3905888	-	480	WP_065805886.1	DNA repair protein RadC	-
LOM31_RS19305 (3905900)	3905900..3906343	-	444	WP_065805885.1	antirestriction protein	-
LOM31_RS19310 (3906423)	3906423..3906653	-	231	WP_087776359.1	DUF905 domain-containing protein	-
LOM31_RS19315 (3906677)	3906677..3907501	-	825	WP_080884212.1	DUF932 domain-containing protein	-
LOM31_RS19320 (3908039)	3908039..3908671	+	633	WP_072032909.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14321.40 Da
• Isoelectric Point: 9.9883

>T225925 WP_065805889.1 NZ_CP087669:c3904733-3904344 [Klebsiella pneumoniae]
MQTKSSPLMRAASSRPSPVSVWQTLLTSLLAQHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T225925 NZ_CP121580:c757-470 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12054.78 Da
• Isoelectric Point: 6.9459

>AT225925 WP_175364756.1 NZ_CP087669:c3905116-3904790 [Klebsiella pneumoniae]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANVSGQFCDADLRHLDQAFPILLKQLELMLTSGELNPRHQHCVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT225925 NZ_CP121580:c483-169 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A2I8S4E2