Detailed information of TA system TA225910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1..475
• Replicon: plasmid pDD01845-5
• Accession: NZ_CP087668
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01845

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: LMH56_RS29680
• Protein ID: WP_004197762.1
• Coordinates: 209..475 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: LMH56_RS29675
• Protein ID: WP_000879771.1
• Coordinates: 1..225 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH56_RS29675 (LMH56_29675)	1..225	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
LMH56_RS29680 (LMH56_29680)	209..475	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH56_RS29685 (LMH56_29685)	545..895	-	351	WP_004197756.1	hypothetical protein	-
LMH56_RS29690 (LMH56_29690)	961..1506	-	546	WP_023317533.1	hypothetical protein	-
LMH56_RS29695 (LMH56_29695)	1532..1858	-	327	WP_050516389.1	hypothetical protein	-
LMH56_RS29700 (LMH56_29700)	3014..3190	+	177	WP_004197757.1	hypothetical protein	-
LMH56_RS29705 (LMH56_29705)	3180..3524	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
LMH56_RS29710 (LMH56_29710)	3733..4167	+	435	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
LMH56_RS29715 (LMH56_29715)	4202..4711	+	510	WP_032433972.1	MbeB family mobilization protein	-
LMH56_RS29720 (LMH56_29720)	4718..4930	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T225910 WP_004197762.1 NZ_CP087668:209-475 [Klebsiella pneumoniae subsp. pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T225910 NZ_CP121560:3934114-3934503 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT225910 WP_000879771.1 NZ_CP087668:1-225 [Klebsiella pneumoniae subsp. pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT225910 NZ_CP121560:3933850-3934107 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5