Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 5864..6389 | Replicon | plasmid pDD01845-2 |
| Accession | NZ_CP087665 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain DD01845 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | D4HQE7 |
| Locus tag | LMH56_RS27965 | Protein ID | WP_013023785.1 |
| Coordinates | 5864..6169 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | W8V2V6 |
| Locus tag | LMH56_RS27970 | Protein ID | WP_001568025.1 |
| Coordinates | 6171..6389 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LMH56_RS27935 (LMH56_27935) | 1567..2193 | + | 627 | WP_004197649.1 | ParA family plasmid-partitioning AAA ATPase | - |
| LMH56_RS27940 (LMH56_27940) | 2190..2492 | + | 303 | WP_004197636.1 | hypothetical protein | - |
| LMH56_RS27945 (LMH56_27945) | 2940..3734 | - | 795 | WP_004197635.1 | site-specific integrase | - |
| LMH56_RS27950 (LMH56_27950) | 3932..4948 | - | 1017 | WP_017899884.1 | hypothetical protein | - |
| LMH56_RS27955 (LMH56_27955) | 4959..5273 | - | 315 | WP_053389906.1 | hypothetical protein | - |
| LMH56_RS27960 (LMH56_27960) | 5300..5695 | - | 396 | WP_017899885.1 | hypothetical protein | - |
| LMH56_RS27965 (LMH56_27965) | 5864..6169 | - | 306 | WP_013023785.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| LMH56_RS27970 (LMH56_27970) | 6171..6389 | - | 219 | WP_001568025.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| LMH56_RS27975 (LMH56_27975) | 7207..7497 | - | 291 | WP_013023783.1 | hypothetical protein | - |
| LMH56_RS27980 (LMH56_27980) | 7494..8621 | - | 1128 | WP_013023782.1 | DUF4238 domain-containing protein | - |
| LMH56_RS27985 (LMH56_27985) | 8655..10178 | - | 1524 | WP_223174996.1 | hypothetical protein | - |
| LMH56_RS27990 (LMH56_27990) | 10454..11233 | - | 780 | WP_013023780.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / mph(A) / tet(A) / floR / blaTEM-1B / blaCTX-M-3 / qnrS1 | - | 1..141469 | 141469 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11615.30 Da Isoelectric Point: 6.4672
>T225905 WP_013023785.1 NZ_CP087665:c6169-5864 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T225905 NZ_CP121529:1150122-1150301 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8392.34 Da Isoelectric Point: 4.7405
>AT225905 WP_001568025.1 NZ_CP087665:c6389-6171 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT225905 NZ_CP121529:1149499-1150098 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZP8 |