Detailed information of TA system TA225904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 143907..144577
• Replicon: plasmid pDD01845-1
• Accession: NZ_CP087664
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01845

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LMH56_RS27530
• Protein ID: WP_004213072.1
• Coordinates: 143907..144350 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LMH56_RS27535
• Protein ID: WP_004213073.1
• Coordinates: 144347..144577 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH56_RS27495 (LMH56_27495)	139318..139593	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LMH56_RS27500 (LMH56_27500)	139656..140147	+	492	WP_011251327.1	DM13 domain-containing protein	-
LMH56_RS27505 (LMH56_27505)	140196..141116	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LMH56_RS27510 (LMH56_27510)	141207..141610	+	404	Protein_154	GAF domain-containing protein	-
LMH56_RS27515 (LMH56_27515)	142128..142763	-	636	Protein_155	mucoid phenotype regulator RmpA2	-
LMH56_RS27520 (LMH56_27520)	143180..143484	+	305	Protein_156	transposase	-
LMH56_RS27525 (LMH56_27525)	143507..143758	-	252	WP_186987481.1	hypothetical protein	-
LMH56_RS27530 (LMH56_27530)	143907..144350	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LMH56_RS27535 (LMH56_27535)	144347..144577	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LMH56_RS27540 (LMH56_27540)	145185..146318	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LMH56_RS27545 (LMH56_27545)	146334..146627	+	294	WP_004213076.1	hypothetical protein	-
LMH56_RS27550 (LMH56_27550)	146617..146823	-	207	WP_004213077.1	hypothetical protein	-
LMH56_RS27555 (LMH56_27555)	147175..147465	+	291	WP_004213078.1	hypothetical protein	-
LMH56_RS27560 (LMH56_27560)	147455..148354	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..219069	219069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T225904 WP_004213072.1 NZ_CP087664:c144350-143907 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T225904 NZ_CP121529:1001422-1001784 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT225904 WP_004213073.1 NZ_CP087664:c144577-144347 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT225904 NZ_CP121529:1001255-1001425 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620