Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4779255..4780065 Replicon chromosome
Accession NZ_CP087663
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01845

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag LMH56_RS23630 Protein ID WP_002887280.1
Coordinates 4779255..4779788 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LMH56_RS23635 Protein ID WP_002887278.1
Coordinates 4779799..4780065 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH56_RS23625 (LMH56_23625) 4778086..4779207 + 1122 WP_002887282.1 cupin domain-containing protein -
LMH56_RS23630 (LMH56_23630) 4779255..4779788 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
LMH56_RS23635 (LMH56_23635) 4779799..4780065 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LMH56_RS23640 (LMH56_23640) 4780168..4781601 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
LMH56_RS23645 (LMH56_23645) 4781591..4782274 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LMH56_RS23650 (LMH56_23650) 4782446..4783831 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
LMH56_RS23655 (LMH56_23655) 4783849..4784193 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T225898 WP_002887280.1 NZ_CP087663:c4779788-4779255 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T225898 NZ_CP121526:c1496285-1496076 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT225898 WP_002887278.1 NZ_CP087663:c4780065-4779799 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT225898 NZ_CP121526:c1496585-1496292 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References