Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4176538..4177157 Replicon chromosome
Accession NZ_CP087663
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01845

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LMH56_RS20745 Protein ID WP_002892050.1
Coordinates 4176939..4177157 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LMH56_RS20740 Protein ID WP_002892066.1
Coordinates 4176538..4176912 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH56_RS20730 (LMH56_20730) 4171690..4172883 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LMH56_RS20735 (LMH56_20735) 4172906..4176052 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LMH56_RS20740 (LMH56_20740) 4176538..4176912 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LMH56_RS20745 (LMH56_20745) 4176939..4177157 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LMH56_RS20750 (LMH56_20750) 4177316..4177882 + 567 WP_002892030.1 maltose O-acetyltransferase -
LMH56_RS20755 (LMH56_20755) 4177854..4177994 - 141 WP_004147370.1 hypothetical protein -
LMH56_RS20760 (LMH56_20760) 4178015..4178485 + 471 WP_002892026.1 YlaC family protein -
LMH56_RS20765 (LMH56_20765) 4178460..4179911 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LMH56_RS20770 (LMH56_20770) 4180012..4180710 + 699 WP_002892021.1 GNAT family protein -
LMH56_RS20775 (LMH56_20775) 4180707..4180847 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LMH56_RS20780 (LMH56_20780) 4180847..4181110 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225897 WP_002892050.1 NZ_CP087663:4176939-4177157 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T225897 NZ_CP121526:c1485235-1484873 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT225897 WP_002892066.1 NZ_CP087663:4176538-4176912 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT225897 NZ_CP121526:c1485402-1485232 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References