Detailed information of TA system TA225880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5872..6397
• Replicon: plasmid pDD01635-2
• Accession: NZ_CP087659
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01635

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: LMH51_RS28445
• Protein ID: WP_013023785.1
• Coordinates: 5872..6177 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: LMH51_RS28450
• Protein ID: WP_001568025.1
• Coordinates: 6179..6397 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH51_RS28415 (LMH51_28415)	1575..2201	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
LMH51_RS28420 (LMH51_28420)	2198..2500	+	303	WP_004197636.1	hypothetical protein	-
LMH51_RS28425 (LMH51_28425)	2948..3742	-	795	WP_004197635.1	site-specific integrase	-
LMH51_RS28430 (LMH51_28430)	3940..4956	-	1017	WP_017899884.1	hypothetical protein	-
LMH51_RS28435 (LMH51_28435)	4967..5281	-	315	WP_053389906.1	hypothetical protein	-
LMH51_RS28440 (LMH51_28440)	5308..5703	-	396	WP_017899885.1	hypothetical protein	-
LMH51_RS28445 (LMH51_28445)	5872..6177	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
LMH51_RS28450 (LMH51_28450)	6179..6397	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LMH51_RS28455 (LMH51_28455)	7215..7505	-	291	WP_013023783.1	hypothetical protein	-
LMH51_RS28460 (LMH51_28460)	7502..8629	-	1128	WP_013023782.1	DUF4238 domain-containing protein	-
LMH51_RS28465 (LMH51_28465)	8663..10186	-	1524	WP_223174996.1	hypothetical protein	-
LMH51_RS28470 (LMH51_28470)	10462..11241	-	780	WP_013023780.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / sul1 / mph(A) / tet(A) / floR / blaTEM-1B / blaCTX-M-3 / qnrS1	-	1..118186	118186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T225880 WP_013023785.1 NZ_CP087659:c6177-5872 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T225880 NZ_CP087659:c6177-5872 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT225880 WP_001568025.1 NZ_CP087659:c6397-6179 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT225880 NZ_CP087659:c6397-6179 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8