Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5430035..5430660 Replicon chromosome
Accession NZ_CP087657
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01635

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LMH51_RS26860 Protein ID WP_002882817.1
Coordinates 5430035..5430418 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LMH51_RS26865 Protein ID WP_004150355.1
Coordinates 5430418..5430660 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH51_RS26845 (LMH51_26845) 5427401..5428303 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LMH51_RS26850 (LMH51_26850) 5428300..5428935 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LMH51_RS26855 (LMH51_26855) 5428932..5429861 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LMH51_RS26860 (LMH51_26860) 5430035..5430418 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH51_RS26865 (LMH51_26865) 5430418..5430660 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LMH51_RS26870 (LMH51_26870) 5430865..5431782 + 918 WP_004181612.1 alpha/beta hydrolase -
LMH51_RS26875 (LMH51_26875) 5431796..5432737 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LMH51_RS26880 (LMH51_26880) 5432782..5433219 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LMH51_RS26885 (LMH51_26885) 5433216..5434076 - 861 WP_004146232.1 virulence factor BrkB family protein -
LMH51_RS26890 (LMH51_26890) 5434070..5434669 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T225876 WP_002882817.1 NZ_CP087657:c5430418-5430035 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T225876 NZ_CP121507:1091371-1091733 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT225876 WP_004150355.1 NZ_CP087657:c5430660-5430418 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT225876 NZ_CP121507:1091204-1091374 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References