Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4851217..4852027 Replicon chromosome
Accession NZ_CP087657
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01635

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag LMH51_RS24105 Protein ID WP_002887280.1
Coordinates 4851217..4851750 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LMH51_RS24110 Protein ID WP_002887278.1
Coordinates 4851761..4852027 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH51_RS24100 (LMH51_24100) 4850048..4851169 + 1122 WP_002887282.1 cupin domain-containing protein -
LMH51_RS24105 (LMH51_24105) 4851217..4851750 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
LMH51_RS24110 (LMH51_24110) 4851761..4852027 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LMH51_RS24115 (LMH51_24115) 4852130..4853563 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
LMH51_RS24120 (LMH51_24120) 4853553..4854236 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LMH51_RS24125 (LMH51_24125) 4854408..4855793 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
LMH51_RS24130 (LMH51_24130) 4855811..4856155 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T225873 WP_002887280.1 NZ_CP087657:c4851750-4851217 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T225873 NZ_CP121502:c1429514-1429152 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT225873 WP_002887278.1 NZ_CP087657:c4852027-4851761 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT225873 NZ_CP121502:c1429681-1429511 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTTCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References