Detailed information of TA system TA225860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 344854..345440
• Replicon: chromosome
• Accession: NZ_CP087657
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01635

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LMH51_RS01610
• Protein ID: WP_002920800.1
• Coordinates: 345072..345440 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: LMH51_RS01605
• Protein ID: WP_004174006.1
• Coordinates: 344854..345075 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH51_RS01585 (LMH51_01585)	341011..341937	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LMH51_RS01590 (LMH51_01590)	341934..343211	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
LMH51_RS01595 (LMH51_01595)	343208..343975	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LMH51_RS01600 (LMH51_01600)	343977..344690	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LMH51_RS01605 (LMH51_01605)	344854..345075	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LMH51_RS01610 (LMH51_01610)	345072..345440	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LMH51_RS01615 (LMH51_01615)	345713..347029	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LMH51_RS01620 (LMH51_01620)	347136..348023	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LMH51_RS01625 (LMH51_01625)	348020..348865	+	846	WP_004181473.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LMH51_RS01630 (LMH51_01630)	348867..349937	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	305217..350674	45457

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T225860 WP_002920800.1 NZ_CP087657:345072-345440 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T225860 NZ_CP121483:1142766-1142954 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT225860 WP_004174006.1 NZ_CP087657:344854-345075 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT225860 NZ_CP121483:1142484-1142741 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAACTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7