Detailed information of TA system TA225859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1502..1976
• Replicon: plasmid pDD01653-5
• Accession: NZ_CP087656
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01653

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: LL272_RS29665
• Protein ID: WP_004197762.1
• Coordinates: 1710..1976 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: LL272_RS29660
• Protein ID: WP_000879771.1
• Coordinates: 1502..1726 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL272_RS29650 (LL272_29650)	107..616	+	510	WP_032433972.1	MbeB family mobilization protein	-
LL272_RS29655 (LL272_29655)	623..835	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
LL272_RS29660 (LL272_29660)	1502..1726	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
LL272_RS29665 (LL272_29665)	1710..1976	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LL272_RS29670 (LL272_29670)	2046..2396	-	351	WP_004197756.1	hypothetical protein	-
LL272_RS29675 (LL272_29675)	2462..3007	-	546	WP_023317533.1	hypothetical protein	-
LL272_RS29680 (LL272_29680)	3033..3359	-	327	WP_050516389.1	hypothetical protein	-
LL272_RS29685 (LL272_29685)	4515..4691	+	177	WP_004197757.1	hypothetical protein	-
LL272_RS29690 (LL272_29690)	4681..5025	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
LL272_RS29695 (LL272_29695)	5207..5452	-	246	WP_032433971.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T225859 WP_004197762.1 NZ_CP087656:1710-1976 [Klebsiella pneumoniae subsp. pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T225859 NZ_CP121483:152692-152871 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT225859 WP_000879771.1 NZ_CP087656:1502-1726 [Klebsiella pneumoniae subsp. pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT225859 NZ_CP121483:152069-152668 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAATTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAACCTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5