Detailed information of TA system TA225858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 3421..4001
• Replicon: plasmid pDD01653-4
• Accession: NZ_CP087655
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01653

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LL272_RS29605
• Protein ID: WP_071177730.1
• Coordinates: 3421..3735 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LL272_RS29610
• Protein ID: WP_000093040.1
• Coordinates: 3723..4001 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL272_RS29585 (LL272_29585)	1329..2060	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
LL272_RS29590 (LL272_29590)	2067..2597	+	531	WP_071177729.1	hypothetical protein	-
LL272_RS29595 (LL272_29595)	2624..2803	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LL272_RS29600 (LL272_29600)	2829..3257	-	429	WP_001140599.1	hypothetical protein	-
LL272_RS29605 (LL272_29605)	3421..3735	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LL272_RS29610 (LL272_29610)	3723..4001	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LL272_RS29615 (LL272_29615)	4176..4541	-	366	WP_072354022.1	TonB family protein	-
LL272_RS29620 (LL272_29620)	4538..4909	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
LL272_RS29625 (LL272_29625)	5183..5428	-	246	WP_032440458.1	hypothetical protein	-
LL272_RS29630 (LL272_29630)	6073..7560	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T225858 WP_071177730.1 NZ_CP087655:c3735-3421 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T225858 NZ_CP121480:2464029-2464391 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT225858 WP_000093040.1 NZ_CP087655:c4001-3723 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT225858 NZ_CP121480:2463862-2464032 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1