Detailed information of TA system TA225852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 145115..145785
• Replicon: plasmid pDD01653-1
• Accession: NZ_CP087652
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01653

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LL272_RS27710
• Protein ID: WP_004213072.1
• Coordinates: 145115..145558 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LL272_RS27715
• Protein ID: WP_004213073.1
• Coordinates: 145555..145785 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL272_RS27675 (LL272_27675)	140526..140801	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LL272_RS27680 (LL272_27680)	140864..141355	+	492	WP_011251327.1	DM13 domain-containing protein	-
LL272_RS27685 (LL272_27685)	141404..142324	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LL272_RS27690 (LL272_27690)	142415..142818	+	404	Protein_155	GAF domain-containing protein	-
LL272_RS27695 (LL272_27695)	143336..143971	-	636	Protein_156	mucoid phenotype regulator RmpA2	-
LL272_RS27700 (LL272_27700)	144388..144692	+	305	Protein_157	transposase	-
LL272_RS27705 (LL272_27705)	144715..144966	-	252	WP_186987481.1	hypothetical protein	-
LL272_RS27710 (LL272_27710)	145115..145558	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LL272_RS27715 (LL272_27715)	145555..145785	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LL272_RS27720 (LL272_27720)	146393..147526	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LL272_RS27725 (LL272_27725)	147542..147835	+	294	WP_004213076.1	hypothetical protein	-
LL272_RS27730 (LL272_27730)	147825..148031	-	207	WP_004213077.1	hypothetical protein	-
LL272_RS27735 (LL272_27735)	148383..148673	+	291	WP_004213078.1	hypothetical protein	-
LL272_RS27740 (LL272_27740)	148663..149562	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..220277	220277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T225852 WP_004213072.1 NZ_CP087652:c145558-145115 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T225852 NZ_CP121477:8082-8291 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT225852 WP_004213073.1 NZ_CP087652:c145785-145555 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT225852 NZ_CP121477:7782-8075 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620