Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4195341..4195960 Replicon chromosome
Accession NZ_CP087651
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01653

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LL272_RS20855 Protein ID WP_002892050.1
Coordinates 4195742..4195960 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LL272_RS20850 Protein ID WP_002892066.1
Coordinates 4195341..4195715 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL272_RS20840 (LL272_20840) 4190493..4191686 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LL272_RS20845 (LL272_20845) 4191709..4194855 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LL272_RS20850 (LL272_20850) 4195341..4195715 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LL272_RS20855 (LL272_20855) 4195742..4195960 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LL272_RS20860 (LL272_20860) 4196119..4196685 + 567 WP_002892030.1 maltose O-acetyltransferase -
LL272_RS20865 (LL272_20865) 4196657..4196797 - 141 WP_004147370.1 hypothetical protein -
LL272_RS20870 (LL272_20870) 4196818..4197288 + 471 WP_002892026.1 YlaC family protein -
LL272_RS20875 (LL272_20875) 4197263..4198714 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LL272_RS20880 (LL272_20880) 4198815..4199513 + 699 WP_002892021.1 GNAT family protein -
LL272_RS20885 (LL272_20885) 4199510..4199650 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LL272_RS20890 (LL272_20890) 4199650..4199913 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225845 WP_002892050.1 NZ_CP087651:4195742-4195960 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T225845 NZ_CP121464:c1523389-1523090 [Janthinobacterium rivuli]
GTGAAAAGCATCCATACCACCGCCACGTTCGATGACTGGTTCAGCCAGTTGAAGGATCGGAAAGCTTTTTTCCGCATCCA
GGCCCGTATTGACCGTGCCGAAGACGGCAACTTTGGCGATTGCAAACCTGTCGGTGGCGGCGTTTCGGAAATGCGAATTG
ATTGTGGTCCCGGTTACCGCGTTTATTTCACCCAGCGCGGCATGGAAATAATCATCCTGCTCGCTGGCGGCGACAAATCA
ACGCAAGCAAAAGATATCAAAACGGCGCAGCGGCTCGCGCAAATACTCCAGGAGGTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT225845 WP_002892066.1 NZ_CP087651:4195341-4195715 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT225845 NZ_CP121464:c1523090-1522794 [Janthinobacterium rivuli]
ATGATACAAAAACTCAACAAGTGGGATAGCGCGCAGCACCTCAAAACCGAAGAGGATATCGTCGCCTACTTTGAAGCTTG
CCTGGAAGAAGCGGGCGACGACGCATCCTTCATCGCCAAAGCGCTCGGCAACATTGCCAAGGCGCGCGGCATGACGCAAC
TTGCCAAGGATACCGGATTGGGACGGGAAAGTTTGTACAAGGCCTTGTCCGGCGAAGGCAATCCAAGCTTCGACACCATT
TTGAAGGTGACGCATGCACTCGGCTTGCAATTTCGCGCCGCAGCGCTGAACACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References