Detailed information of TA system TA225832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1309..1783
• Replicon: plasmid pDD01754-5
• Accession: NZ_CP087650
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01754

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: LMH55_RS30180
• Protein ID: WP_004197762.1
• Coordinates: 1517..1783 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: LMH55_RS30175
• Protein ID: WP_000879771.1
• Coordinates: 1309..1533 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH55_RS30170 (LMH55_30170)	430..642	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
LMH55_RS30175 (LMH55_30175)	1309..1533	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
LMH55_RS30180 (LMH55_30180)	1517..1783	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH55_RS30185 (LMH55_30185)	1853..2203	-	351	WP_004197756.1	hypothetical protein	-
LMH55_RS30190 (LMH55_30190)	2269..2814	-	546	WP_023317533.1	hypothetical protein	-
LMH55_RS30195 (LMH55_30195)	2840..3166	-	327	WP_050516389.1	hypothetical protein	-
LMH55_RS30200 (LMH55_30200)	4322..4498	+	177	WP_004197757.1	hypothetical protein	-
LMH55_RS30205 (LMH55_30205)	4488..4832	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
LMH55_RS30210 (LMH55_30210)	5014..5259	-	246	WP_032433971.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T225832 WP_004197762.1 NZ_CP087650:1517-1783 [Klebsiella pneumoniae subsp. pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T225832 NZ_CP121375:c3255022-3254633 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTACACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAAAGTGGAAAAAGCTTAAAACTTTAGAAAAACTTTATTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT225832 WP_000879771.1 NZ_CP087650:1309-1533 [Klebsiella pneumoniae subsp. pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT225832 NZ_CP121375:c3255286-3255029 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACTTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5