Detailed information of TA system TA225831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 5952..6532
• Replicon: plasmid pDD01754-4
• Accession: NZ_CP087649
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01754

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LMH55_RS30135
• Protein ID: WP_071177730.1
• Coordinates: 5952..6266 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LMH55_RS30140
• Protein ID: WP_000093040.1
• Coordinates: 6254..6532 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH55_RS30110 (LMH55_30110)	1896..3860	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
LMH55_RS30115 (LMH55_30115)	3860..4591	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
LMH55_RS30120 (LMH55_30120)	4598..5128	+	531	WP_071177729.1	hypothetical protein	-
LMH55_RS30125 (LMH55_30125)	5155..5334	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LMH55_RS30130 (LMH55_30130)	5360..5788	-	429	WP_001140599.1	hypothetical protein	-
LMH55_RS30135 (LMH55_30135)	5952..6266	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH55_RS30140 (LMH55_30140)	6254..6532	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LMH55_RS30145 (LMH55_30145)	6707..7072	-	366	WP_072354022.1	TonB family protein	-
LMH55_RS30150 (LMH55_30150)	7069..7440	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
LMH55_RS30155 (LMH55_30155)	7714..7959	-	246	WP_032440458.1	hypothetical protein	-
LMH55_RS30160 (LMH55_30160)	8286..9971	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10061	10061

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T225831 WP_071177730.1 NZ_CP087649:c6266-5952 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T225831 NZ_CP121375:c2187386-2187204 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT225831 WP_000093040.1 NZ_CP087649:c6532-6254 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT225831 NZ_CP121375:c2187111-2186707 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1