Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 104430..105073 Replicon plasmid pDD01754-2
Accession NZ_CP087647
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01754

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LMH55_RS29250 Protein ID WP_001044770.1
Coordinates 104430..104846 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LMH55_RS29255 Protein ID WP_001261282.1
Coordinates 104843..105073 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH55_RS29230 (100533) 100533..100805 - 273 Protein_124 transposase -
LMH55_RS29240 (101787) 101787..102809 - 1023 WP_000361404.1 helicase UvrD -
LMH55_RS29245 (102794) 102794..104356 - 1563 WP_004206609.1 AAA family ATPase -
LMH55_RS29250 (104430) 104430..104846 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH55_RS29255 (104843) 104843..105073 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LMH55_RS29260 (105030) 105030..105491 + 462 WP_014343465.1 hypothetical protein -
LMH55_RS29265 (105652) 105652..106596 + 945 WP_011977810.1 hypothetical protein -
LMH55_RS29270 (106633) 106633..107025 + 393 WP_011977811.1 hypothetical protein -
LMH55_RS29275 (107083) 107083..107604 + 522 WP_013214008.1 hypothetical protein -
LMH55_RS29280 (107650) 107650..107853 + 204 WP_011977813.1 hypothetical protein -
LMH55_RS29285 (107883) 107883..108887 + 1005 WP_011977814.1 hypothetical protein -
LMH55_RS29290 (109071) 109071..109850 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2 - 1..182556 182556


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T225829 WP_001044770.1 NZ_CP087647:c104846-104430 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T225829 NZ_CP121375:c111499-111236 [Acinetobacter baumannii]
ATGACGAGTCGTAATGTCGAATGGACTGAAAATGCCTGGGATGAATATATCTATTGGCAAACACAGGATAAAAAGATACT
CAAACGTATCAATACCTTAATCAGAGAATGTCAAAGAACACCCTTTGAAGGAACTGGGAAACCAGAACCTTTAAAAGCAA
ATCTTTCAGGATTCTGGAGCCGTAGAATTGATGAGAAGCATAGATTAGTTTATGAAGTGACGGATGAACGAATTTCCATT
ATTCAATGTCGGTTCCATTACTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT225829 WP_001261282.1 NZ_CP087647:c105073-104843 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT225829 NZ_CP121375:c111749-111486 [Acinetobacter baumannii]
ATGCATGTATTTACATATACAGACGCACGTAACAACTTAAAATCTGTTTTAGATAAAGTTATTGATGATGCAGATGTCGC
TTTCATTACAAGAAAAGAAGGCGGTCATGCTGTAGTAATGGGACAAGATCATTACGATAGTTTAATGGAAACGTTGTATC
TCCTTTCATCTCCAAATAATACCACTCGTTTAAACGAATCAATTGCTCAATTACGCGCAGGTAAAACCAAACAAAGAGAG
TTGATTAAAGATGACGAGTCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References