Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5402805..5403430 Replicon chromosome
Accession NZ_CP087645
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01754

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LMH55_RS26705 Protein ID WP_002882817.1
Coordinates 5402805..5403188 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LMH55_RS26710 Protein ID WP_004150355.1
Coordinates 5403188..5403430 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH55_RS26690 (LMH55_26690) 5400171..5401073 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LMH55_RS26695 (LMH55_26695) 5401070..5401705 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LMH55_RS26700 (LMH55_26700) 5401702..5402631 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LMH55_RS26705 (LMH55_26705) 5402805..5403188 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH55_RS26710 (LMH55_26710) 5403188..5403430 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LMH55_RS26715 (LMH55_26715) 5403635..5404552 + 918 WP_002882812.1 alpha/beta hydrolase -
LMH55_RS26720 (LMH55_26720) 5404566..5405507 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LMH55_RS26725 (LMH55_26725) 5405552..5405989 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LMH55_RS26730 (LMH55_26730) 5405986..5406846 - 861 WP_002882807.1 virulence factor BrkB family protein -
LMH55_RS26735 (LMH55_26735) 5406840..5407439 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T225824 WP_002882817.1 NZ_CP087645:c5403188-5402805 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T225824 NZ_CP121371:c16504-16208 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTGCCTGAATTTGATGAATGGCTAGATGGTCTTAAAGACAATATGACTCGAATCCGCTTGAA
TCGTCGATTAGATAAGGTCCAACGTGGTAATTGGGGTGACATTAAACCACTCCAAGATGGTGTCTGGGAAATGAGAGAGT
TCTTTGGTGCTGGTTGGCGGATGTACTACATCCAACATGGTGACGTTGTGATCGTGATGCTTGGTGGTGGTGAAAAATCT
ACACAGAAAGATGACATCAAGAAAGCGGTCAAACTATCGAAAACATTGGAGGATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT225824 WP_004150355.1 NZ_CP087645:c5403430-5403188 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT225824 NZ_CP121371:c16206-15916 [Acinetobacter baumannii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGATATTGTAATGTACCTCAA
TATGGTTCTTGAAGAAAATGATCCTGCTGAATTAGCGCACGCACTTGGTGTAATTGCTAAAGCAAGAGGTATGACCCAAA
TTGCGAAAGAAGCAGGTATTGGACGAGAAGCCCTTTACAAAGCACTTCGTCAAGACTCAACGCCTCGTTTTGATACGATC
AACCGTGTTGTGAATGCTTTAGGTTTAAAATTGACAGTTCAACATACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References