Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4815465..4816275 Replicon chromosome
Accession NZ_CP087645
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01754

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag LMH55_RS23880 Protein ID WP_002887280.1
Coordinates 4815465..4815998 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LMH55_RS23885 Protein ID WP_002887278.1
Coordinates 4816009..4816275 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH55_RS23875 (LMH55_23875) 4814296..4815417 + 1122 WP_002887282.1 cupin domain-containing protein -
LMH55_RS23880 (LMH55_23880) 4815465..4815998 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
LMH55_RS23885 (LMH55_23885) 4816009..4816275 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LMH55_RS23890 (LMH55_23890) 4816378..4817811 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
LMH55_RS23895 (LMH55_23895) 4817801..4818484 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LMH55_RS23900 (LMH55_23900) 4818656..4820041 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
LMH55_RS23905 (LMH55_23905) 4820059..4820403 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T225821 WP_002887280.1 NZ_CP087645:c4815998-4815465 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T225821 NZ_CP121370:c2185626-2185444 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT225821 WP_002887278.1 NZ_CP087645:c4816275-4816009 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT225821 NZ_CP121370:c2185351-2184947 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References