Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4201683..4202302 Replicon chromosome
Accession NZ_CP087645
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01754

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LMH55_RS20930 Protein ID WP_002892050.1
Coordinates 4202084..4202302 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LMH55_RS20925 Protein ID WP_002892066.1
Coordinates 4201683..4202057 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH55_RS20915 (LMH55_20915) 4196835..4198028 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LMH55_RS20920 (LMH55_20920) 4198051..4201197 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LMH55_RS20925 (LMH55_20925) 4201683..4202057 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LMH55_RS20930 (LMH55_20930) 4202084..4202302 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LMH55_RS20935 (LMH55_20935) 4202461..4203027 + 567 WP_002892030.1 maltose O-acetyltransferase -
LMH55_RS20940 (LMH55_20940) 4202999..4203139 - 141 WP_004147370.1 hypothetical protein -
LMH55_RS20945 (LMH55_20945) 4203160..4203630 + 471 WP_002892026.1 YlaC family protein -
LMH55_RS20950 (LMH55_20950) 4203605..4205056 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LMH55_RS20955 (LMH55_20955) 4205157..4205855 + 699 WP_002892021.1 GNAT family protein -
LMH55_RS20960 (LMH55_20960) 4205852..4205992 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LMH55_RS20965 (LMH55_20965) 4205992..4206255 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225820 WP_002892050.1 NZ_CP087645:4202084-4202302 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T225820 NZ_CP121370:c110849-110586 [Acinetobacter baumannii]
ATGCATGTATTTACATATACAGACGCACGTAACAACTTAAAATCTGTTTTAGATAAAGTTATTGATGATGCAGATGTCGC
TTTCATTACAAGAAAAGAAGGCGGTCATGCTGTAGTAATGGGACAAGATCATTACGATAGTTTAATGGAAACGTTGTATC
TCCTTTCATCTCCAAATAATACCACTCGTTTAAACGAATCAATTGCTCAATTACGCGCAGGTAAAACCAAACAAAGAGAG
TTGATTAAAGATGACGAGTCGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT225820 WP_002892066.1 NZ_CP087645:4201683-4202057 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT225820 NZ_CP121370:c110599-110336 [Acinetobacter baumannii]
ATGACGAGTCGTAATGTCGAATGGACTGAAAATGCCTGGGATGAATATATCTATTGGCAAACACAGGATAAAAAGATACT
CAAACGTATCAATACCTTAATCAGAGAATGTCAAAGAACACCCTTTGAAGGAACTGGGAAACCAGAACCTTTAAAAGCAA
ATCTTTCAGGATTCTGGAGCCGTAGAATTGATGAGAAGCATAGATTAGTTTATGAAGTGACGGATGAACGAATTTCCATT
ATTCAATGTCGGTTCCATTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References