Detailed information of TA system TA225807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 331258..331844
• Replicon: chromosome
• Accession: NZ_CP087645
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01754

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LMH55_RS01545
• Protein ID: WP_002920800.1
• Coordinates: 331476..331844 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0H3GZM4
• Locus tag: LMH55_RS01540
• Protein ID: WP_002920802.1
• Coordinates: 331258..331479 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH55_RS01520 (LMH55_01520)	327415..328341	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LMH55_RS01525 (LMH55_01525)	328338..329615	+	1278	WP_002920806.1	branched chain amino acid ABC transporter permease LivM	-
LMH55_RS01530 (LMH55_01530)	329612..330379	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LMH55_RS01535 (LMH55_01535)	330381..331094	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LMH55_RS01540 (LMH55_01540)	331258..331479	+	222	WP_002920802.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LMH55_RS01545 (LMH55_01545)	331476..331844	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LMH55_RS01550 (LMH55_01550)	332117..333433	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LMH55_RS01555 (LMH55_01555)	333540..334427	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LMH55_RS01560 (LMH55_01560)	334424..335269	+	846	WP_002920789.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LMH55_RS01565 (LMH55_01565)	335271..336341	+	1071	WP_002920787.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	326244..337078	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T225807 WP_002920800.1 NZ_CP087645:331476-331844 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T225807 NZ_CP121361:c40685-40311 [Escherichia coli]
ATGATATTAGTAGATACGTCTGTGTGGGTTGATCATTTTAAAAATAAAAATGATACATTGATTCAGCTATTGCAGTCAGA
CTTTGTTCTTATGCACCCCATGATCCTGGCTGAAATTGCATGCGGCACACCGCCAGCGCCCAGGCAGCGAACATTGAGTG
ATTTAGACTTGTTACCGAAAAGTCATCAGGCCACGATCACTGAAGTTCTGGCGTTCATTGAAAATGAAAAATTATTCGGA
CTGGGTTGTGGTCTGGTTGATATAACATTATTAGCGTCAACGAGGATTACCCCCGGGGCTAAAATATGGACGCTGGATAA
GCGACTGTCACGACTGGCAAAACACCTGAATGTAGAATATCAGCCTGTACATTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8181.18 Da
• Isoelectric Point: 4.7769

>AT225807 WP_002920802.1 NZ_CP087645:331258-331479 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT225807 NZ_CP121361:c40885-40682 [Escherichia coli]
ATGAGAACTACTGTAACGATCGACGATGTTCTGTACGCACAGGCGCTGGAAATGGCTGATCCGGGTATGGATAAATCAGA
TATTTTCCGTGAAGCTGTGAAAACGTTTATTCGTGTGCAGGCTGCGAAACGCCTGGCTTCACTTGGTGGAGCTGCCCCGA
ATATGGAGATAACACCACGCAGGCGTGGAGATGTGGCAGAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A0H3GZM4