Detailed information of TA system TA225806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2597..3071
• Replicon: plasmid pDD02391-5
• Accession: NZ_CP087644
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02391

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: LMH57_RS29250
• Protein ID: WP_004197762.1
• Coordinates: 2597..2863 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: LMH57_RS29255
• Protein ID: WP_000879771.1
• Coordinates: 2847..3071 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH57_RS29230 (LMH57_29230)	1..5596	-	5596	WP_004197757.1	hypothetical protein	-
LMH57_RS29235 (LMH57_29235)	1214..1540	+	327	WP_050516389.1	hypothetical protein	-
LMH57_RS29240 (LMH57_29240)	1566..2111	+	546	WP_023317533.1	hypothetical protein	-
LMH57_RS29245 (LMH57_29245)	2177..2527	+	351	WP_004197756.1	hypothetical protein	-
LMH57_RS29250 (LMH57_29250)	2597..2863	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH57_RS29255 (LMH57_29255)	2847..3071	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
LMH57_RS29260 (LMH57_29260)	3738..3950	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
LMH57_RS29265 (LMH57_29265)	3957..4466	-	510	WP_032433972.1	MbeB family mobilization protein	-
LMH57_RS29270 (LMH57_29270)	4501..4935	-	435	Protein_8	relaxase/mobilization nuclease domain-containing protein	-
LMH57_RS29275 (LMH57_29275)	5144..5488	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T225806 WP_004197762.1 NZ_CP087644:c2863-2597 [Klebsiella pneumoniae subsp. pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T225806 NZ_CP121360:92695-92982 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGTGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT225806 WP_000879771.1 NZ_CP087644:c3071-2847 [Klebsiella pneumoniae subsp. pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT225806 NZ_CP121360:92447-92698 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TTGATGCCCTAGACGATCAGGAGTTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGATCTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5