Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18826..19469 Replicon plasmid pDD02391-2
Accession NZ_CP087641
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02391

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LMH57_RS28035 Protein ID WP_001044770.1
Coordinates 19053..19469 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LMH57_RS28030 Protein ID WP_001261282.1
Coordinates 18826..19056 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH57_RS28005 (LMH57_28005) 14766..14933 - 168 Protein_17 IS1 family transposase -
LMH57_RS28010 (LMH57_28010) 15237..16166 + 930 WP_004213558.1 Rpn family recombination-promoting nuclease/putative transposase -
LMH57_RS28015 (LMH57_28015) 16311..17090 - 780 WP_004213560.1 site-specific integrase -
LMH57_RS28020 (LMH57_28020) 17087..17908 - 822 WP_004213562.1 hypothetical protein -
LMH57_RS28025 (LMH57_28025) 18453..18869 - 417 WP_164481821.1 hypothetical protein -
LMH57_RS28030 (LMH57_28030) 18826..19056 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LMH57_RS28035 (LMH57_28035) 19053..19469 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH57_RS28040 (LMH57_28040) 19543..21105 + 1563 WP_004206609.1 AAA family ATPase -
LMH57_RS28045 (LMH57_28045) 21090..22112 + 1023 WP_000361404.1 helicase UvrD -
LMH57_RS28050 (LMH57_28050) 22368..23064 + 697 WP_024269734.1 IS1 family transposase -
LMH57_RS28055 (LMH57_28055) 23093..23365 + 273 Protein_27 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - rmpA / iroN 1..132733 132733
- inside IScluster/Tn - - 22368..47710 25342


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T225803 WP_001044770.1 NZ_CP087641:19053-19469 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T225803 NZ_CP121358:21677-21979 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT225803 WP_001261282.1 NZ_CP087641:18826-19056 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT225803 NZ_CP121358:22050-22388 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References