Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4197111..4197730 Replicon chromosome
Accession NZ_CP087639
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02391

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LMH57_RS20940 Protein ID WP_002892050.1
Coordinates 4197512..4197730 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LMH57_RS20935 Protein ID WP_002892066.1
Coordinates 4197111..4197485 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH57_RS20925 (LMH57_20925) 4192263..4193456 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LMH57_RS20930 (LMH57_20930) 4193479..4196625 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LMH57_RS20935 (LMH57_20935) 4197111..4197485 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LMH57_RS20940 (LMH57_20940) 4197512..4197730 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LMH57_RS20945 (LMH57_20945) 4197889..4198455 + 567 WP_002892030.1 maltose O-acetyltransferase -
LMH57_RS20950 (LMH57_20950) 4198427..4198567 - 141 WP_004147370.1 hypothetical protein -
LMH57_RS20955 (LMH57_20955) 4198588..4199058 + 471 WP_002892026.1 YlaC family protein -
LMH57_RS20960 (LMH57_20960) 4199033..4200484 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LMH57_RS20965 (LMH57_20965) 4200585..4201283 + 699 WP_002892021.1 GNAT family protein -
LMH57_RS20970 (LMH57_20970) 4201280..4201420 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LMH57_RS20975 (LMH57_20975) 4201420..4201683 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225794 WP_002892050.1 NZ_CP087639:4197512-4197730 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T225794 NZ_CP121356:3056044-3056151 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT225794 WP_002892066.1 NZ_CP087639:4197111-4197485 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT225794 NZ_CP121356:c3055997-3055931 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCTGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References