Detailed information of TA system TA225780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1446..1920
• Replicon: plasmid pDD02297-4
• Accession: NZ_CP087638
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02297

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: LMH52_RS27995
• Protein ID: WP_004197762.1
• Coordinates: 1654..1920 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: LMH52_RS27990
• Protein ID: WP_000879771.1
• Coordinates: 1446..1670 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH52_RS27980 (LMH52_27980)	51..560	+	510	WP_032433972.1	MbeB family mobilization protein	-
LMH52_RS27985 (LMH52_27985)	567..779	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
LMH52_RS27990 (LMH52_27990)	1446..1670	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
LMH52_RS27995 (LMH52_27995)	1654..1920	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH52_RS28000 (LMH52_28000)	1990..2340	-	351	WP_004197756.1	hypothetical protein	-
LMH52_RS28005 (LMH52_28005)	2406..2951	-	546	WP_023317533.1	hypothetical protein	-
LMH52_RS28010 (LMH52_28010)	2977..3303	-	327	WP_050516389.1	hypothetical protein	-
LMH52_RS28015 (LMH52_28015)	4459..4635	+	177	WP_004197757.1	hypothetical protein	-
LMH52_RS28020 (LMH52_28020)	4625..4969	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
LMH52_RS28025 (LMH52_28025)	5151..5396	-	246	WP_032433971.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T225780 WP_004197762.1 NZ_CP087638:1654-1920 [Klebsiella pneumoniae subsp. pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T225780 NZ_CP087638:1654-1920 [Klebsiella pneumoniae subsp. pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT225780 WP_000879771.1 NZ_CP087638:1446-1670 [Klebsiella pneumoniae subsp. pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT225780 NZ_CP087638:1446-1670 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5