Detailed information of TA system TA225779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 765..1345
• Replicon: plasmid pDD02297-3
• Accession: NZ_CP087637
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02297

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LMH52_RS27930
• Protein ID: WP_071177730.1
• Coordinates: 1031..1345 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LMH52_RS27925
• Protein ID: WP_000093040.1
• Coordinates: 765..1043 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH52_RS27920 (LMH52_27920)	225..590	+	366	WP_072354022.1	TonB family protein	-
LMH52_RS27925 (LMH52_27925)	765..1043	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LMH52_RS27930 (LMH52_27930)	1031..1345	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH52_RS27935 (LMH52_27935)	1509..1937	+	429	WP_001140599.1	hypothetical protein	-
LMH52_RS27940 (LMH52_27940)	1963..2142	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LMH52_RS27945 (LMH52_27945)	2169..2699	-	531	WP_071177729.1	hypothetical protein	-
LMH52_RS27950 (LMH52_27950)	2706..3437	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
LMH52_RS27955 (LMH52_27955)	3437..5401	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10062	10062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T225779 WP_071177730.1 NZ_CP087637:1031-1345 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T225779 NZ_CP121355:5506074-5506601 [Mesorhizobium sp. WSM4906]
ATGACACTGCCGCGATGGCGCGAGGAGCCGATCTCGAAATCGCATGATCGCGCGTCCTTCGATTGCGGCGACGAACAGAT
GAACGATTTTCTGCGGCGCTTCGCCAGGCAGAGCCATGAACAGAATGCCTCGAAGACATTTTGCGGCGTCGACAACGCGA
ACCCAGACCGCGTTCTTGGCTTCTATACGATCGCACCCTCCGCCGTTGCTCATGAGCAAGTACCCGCTGCCCTGACGAAA
GGGCTGGCCCGACATGAAGTATCCGGATTCAAGCTTGCCCGCCTGGCGACCGATCTCTCGGTGGCCGGGCAAGGGCTTGG
CGGTCAGCTTCTCGCTGCGGCGGCCTTGCGGTGCCTGCGTGTCGCCGGCGAAGGTGGAGGTCTCCTGTTCATCATCGATG
CCAAGAGCGAGCGCGCCGCGAACTGGTACCGTGGATATGGTGCCGAGCCGCTGTCGGGAAAACCGCTGACTCTCGTGATG
CCGCTGGCGACGTTCGCTGCCGATCTCAGGGCTAAAGGCCTGTTATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT225779 WP_000093040.1 NZ_CP087637:765-1043 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT225779 NZ_CP121355:5505799-5506077 [Mesorhizobium sp. WSM4906]
ATGTCCAAGCCAGCCAGCGACAGAACCGAACGCTTCAATATCCGCATACGGCCCGCGCAGAAGGCGATGATCGCCCGCGC
GGCGGCGCTGTCGCACAAGGACATGACCGACTTCATCCTCGACAAGGTGCTGCCTGAGGCGGAAGCCGTCATCAAGGCGA
TCGAAAAGACCGAAGTCTCGGAACGCGACTTCAAACGCATCCTCGAACTGCTCGAGCATCCACCGAAGCCAAATGCGAAA
TTGCGGGCCGCCATTGCAGCTCTTCCCGATACGATATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1