Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 28573..29216 Replicon plasmid pDD02297-1
Accession NZ_CP087635
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02297

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LMH52_RS27320 Protein ID WP_001044770.1
Coordinates 28573..28989 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LMH52_RS27325 Protein ID WP_001261282.1
Coordinates 28986..29216 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH52_RS27300 (24676) 24676..24948 - 273 Protein_33 transposase -
LMH52_RS27310 (25930) 25930..26952 - 1023 WP_000361404.1 helicase UvrD -
LMH52_RS27315 (26937) 26937..28499 - 1563 WP_004206609.1 AAA family ATPase -
LMH52_RS27320 (28573) 28573..28989 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH52_RS27325 (28986) 28986..29216 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LMH52_RS27330 (29173) 29173..29634 + 462 WP_014343465.1 hypothetical protein -
LMH52_RS27335 (29795) 29795..30739 + 945 WP_011977810.1 hypothetical protein -
LMH52_RS27340 (30776) 30776..31168 + 393 WP_011977811.1 hypothetical protein -
LMH52_RS27345 (31226) 31226..31747 + 522 WP_013214008.1 hypothetical protein -
LMH52_RS27350 (31793) 31793..31996 + 204 WP_011977813.1 hypothetical protein -
LMH52_RS27355 (32026) 32026..33030 + 1005 WP_011977814.1 hypothetical protein -
LMH52_RS27360 (33214) 33214..33993 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / blaKPC-2 - 1..76107 76107


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T225776 WP_001044770.1 NZ_CP087635:c28989-28573 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T225776 NZ_CP121355:4551494-4551685 [Mesorhizobium sp. WSM4906]
ATCGAGCGTGACAGCCGTAAAATCGTGAAGCGTCTCAAGGACGACGGTTTCGAGCTGGTATCCGTTCGCGGCTCGCATCA
CAAATTTCGAAAGGGCGAGATCGTGTTGATCGTACCGCATCCGGAGAAGGATCTGCCAATAGGCACTGCTCGCGCAATCG
CCAAGCAAGCAGGCTGGATCAAAACAAACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT225776 WP_001261282.1 NZ_CP087635:c29216-28986 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT225776 NZ_CP121355:4551721-4552119 [Mesorhizobium sp. WSM4906]
GTGAAGAGCTATTTTGCTTTGGTCGACAAAGATGCGGGCAGCGCTTTCGGCATTCGCTTTCCAGACATTCCCGGCTGCTT
TTCAGCCGCGGATGCTGCAGAAGAAATCATTCCGAATGCGGTCGAGGCGTTACAATTGTGGGCAGAGGACATGCCAGTCC
CCGAACCTTCCAGCCACGAGGCGATCGTCTCGCTTTCGGAGGTTCGCACGGCCTTGGCCGAGGGAGCATACTTGGTGTCG
GTGCCGTTGATCGACAATGACAGTGCTGTGGTCAGGGCCAACGTCACGTTTGAGCGTGGCGTCTTGCGAGCGATCGATGC
TGCGGCGCGTGAACGCGGCATAACAAGATCCGCGTTCCTGGCCAGCGCAGCGCGCAAGGAAATCGAAGCCAAGCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References