Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5419258..5419883 Replicon chromosome
Accession NZ_CP087634
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02297

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LMH52_RS26770 Protein ID WP_002882817.1
Coordinates 5419258..5419641 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LMH52_RS26775 Protein ID WP_004150355.1
Coordinates 5419641..5419883 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH52_RS26755 (LMH52_26755) 5416624..5417526 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LMH52_RS26760 (LMH52_26760) 5417523..5418158 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LMH52_RS26765 (LMH52_26765) 5418155..5419084 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LMH52_RS26770 (LMH52_26770) 5419258..5419641 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH52_RS26775 (LMH52_26775) 5419641..5419883 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LMH52_RS26780 (LMH52_26780) 5420088..5421005 + 918 WP_002882812.1 alpha/beta hydrolase -
LMH52_RS26785 (LMH52_26785) 5421019..5421960 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LMH52_RS26790 (LMH52_26790) 5422005..5422442 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LMH52_RS26795 (LMH52_26795) 5422439..5423299 - 861 WP_002882807.1 virulence factor BrkB family protein -
LMH52_RS26800 (LMH52_26800) 5423293..5423892 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T225775 WP_002882817.1 NZ_CP087634:c5419641-5419258 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T225775 NZ_CP121355:4237116-4237370 [Mesorhizobium sp. WSM4906]
ATGAAGCGAAAGCATCAGGCAACGCTTGCGCAGATTTTCTCGCGACCGGTAAGCGGCACAATTCGATGGTCGGATATCGA
AGCCTTGTTCGTGGCTTTGGGTGCTGAAGTCAGCGAACGCGAAGGATCGCGTGTCGGCGTGTTTCTGTTCGGTGAGGTCC
GCGTTTTCCATCGGCCTCATCCGACACCGGACACTGATAAGGGCGCGGTTGCCAGCATCAGGAAATGGCTGGAGAGCCAT
GGAGTGAGACCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT225775 WP_004150355.1 NZ_CP087634:c5419883-5419641 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT225775 NZ_CP121355:4237367-4237705 [Mesorhizobium sp. WSM4906]
ATGAAGAACATCATTGAGATCGACGGCCACAAGGCATCGGTGTCTTTCGACCCGGAGCTGGGAATGCTGAGGGGCGAATT
CCTGGGTCTGGCCGGCGGCGCGGATTTTTACGCCGCGAATGTCGAACAACTCTACGCCGAGGGCGCGGCGTCGTTGAAAG
TTTTCCTCGAAATGTGCCGCGAGAAAGGCATCAAGCCTTTTCGGGAATTTTCCGGCCGCTTCAATGTTCGGCTCGATCCC
GCTACTCATCAGGCCGCCGTTATCGCCGCCGCCGCCGAGAACAAAAGCCTCAACGAGTGGGTTGCCCAAGCGATCGAAAC
GGCCGCTCTGGCCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References