Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4834530..4835340 Replicon chromosome
Accession NZ_CP087634
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02297

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag LMH52_RS23965 Protein ID WP_002887280.1
Coordinates 4834530..4835063 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LMH52_RS23970 Protein ID WP_002887278.1
Coordinates 4835074..4835340 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH52_RS23960 (LMH52_23960) 4833361..4834482 + 1122 WP_002887282.1 cupin domain-containing protein -
LMH52_RS23965 (LMH52_23965) 4834530..4835063 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
LMH52_RS23970 (LMH52_23970) 4835074..4835340 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LMH52_RS23975 (LMH52_23975) 4835443..4836876 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
LMH52_RS23980 (LMH52_23980) 4836866..4837549 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LMH52_RS23985 (LMH52_23985) 4837721..4839106 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
LMH52_RS23990 (LMH52_23990) 4839124..4839468 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T225772 WP_002887280.1 NZ_CP087634:c4835063-4834530 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T225772 NZ_CP121355:1178073-1178369 [Mesorhizobium sp. WSM4906]
ATGATCCTTGGCTTTCGGGACGAGTGGTTGCGAGCCTTCTTCGTTGACGACGCGCATTCCAGGAATATCCCGTCCGATCT
CGAACTGCGGCTGTTCCGCAAACTTCAGATGATCGATGATGCGATGACCGATCCGGACCTGCGCGTGCCGCCCAGCAATC
ATTTCGAGAAGTTGCGCGGCAACCTCGAAGGGTTCCATTCGATCCGTGTCAACAAGCAGTGGCGGCTAGTATTCCGGTGG
GACGGTCGCCGCGGTGAAGCGTCGGACGTCTATCTTGACGACCATAGCTACAGATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT225772 WP_002887278.1 NZ_CP087634:c4835340-4835074 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT225772 NZ_CP121355:1178377-1178685 [Mesorhizobium sp. WSM4906]
ATGCTGGCGACCAAGCGCAGGCCGGTGACGATCGGCGAGATACTGGTGGAGGAATTCATGCGGCCGATGGGCCTCACCCA
AGCCGCTCTAGCGGAGGCGATGGGTGTGCAACGCAAGCATGTCAACGAGCTCTGCAACAATCGTCGCAACGTCACGGCGC
CGACCGCGCTTATCCTTGCACGCGTGTTCGGCAACAGTCCCGAGTTCTGGCTCAATGTGCAACGGCGCAGCGACCTGTGG
GAAGCGATGCACGATCCGAAGGAGCGCGAGAGGATCAAACGGGCGCGCCCGTTGCCCAATGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References