Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4221064..4221683 Replicon chromosome
Accession NZ_CP087634
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02297

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LMH52_RS21015 Protein ID WP_002892050.1
Coordinates 4221465..4221683 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LMH52_RS21010 Protein ID WP_002892066.1
Coordinates 4221064..4221438 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH52_RS21000 (LMH52_21000) 4216216..4217409 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LMH52_RS21005 (LMH52_21005) 4217432..4220578 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LMH52_RS21010 (LMH52_21010) 4221064..4221438 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LMH52_RS21015 (LMH52_21015) 4221465..4221683 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LMH52_RS21020 (LMH52_21020) 4221842..4222408 + 567 WP_002892030.1 maltose O-acetyltransferase -
LMH52_RS21025 (LMH52_21025) 4222380..4222520 - 141 WP_004147370.1 hypothetical protein -
LMH52_RS21030 (LMH52_21030) 4222541..4223011 + 471 WP_002892026.1 YlaC family protein -
LMH52_RS21035 (LMH52_21035) 4222986..4224437 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LMH52_RS21040 (LMH52_21040) 4224538..4225236 + 699 WP_002892021.1 GNAT family protein -
LMH52_RS21045 (LMH52_21045) 4225233..4225373 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LMH52_RS21050 (LMH52_21050) 4225373..4225636 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225771 WP_002892050.1 NZ_CP087634:4221465-4221683 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T225771 NZ_CP121354:5486001-5486528 [Mesorhizobium sp. WSM4904]
ATGACACTGCCGCGATGGCGCGAGGAGCCGATCTCGAAATCGCATGATCGCGCGTCCTTCGATTGCGGCGACGAACAGAT
GAACGATTTTCTGCGGCGCTTCGCCAGGCAGAGCCATGAACAGAATGCCTCGAAGACATTTTGCGCCGTCGACAACGCGA
ACCCGGACCGCGTTCTTGGCTTCTATACGATCGCACCCTCCGCCGTTGCTCATGAGCAAGTGCCTGCGGCCCTGACGAAA
GGGCTGGCCCGACATGAAGTATCCGGATTCAAGCTTGCCCGCCTGGCGACCGATCTCTCGGTGGCCGGGCAAGGGCTTGG
CGGTCAGCTTCTCGCTGCGGCGGCCTTGCGCTGCCTGCGTGTCGCCGGCGAAGGTGGAGGTCTCCTGTTCATCATCGATG
CCAAGAGCGAGCGCGCCGCGAACTGGTACCGTGGATATGGTGCCGAGCCGCTGTCGGGAAAACCGCTGACTCTCGTGATG
CCGCTGGCGACGTTCGCTGCCGATCTCAGGGCTAAAGACCTGTTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT225771 WP_002892066.1 NZ_CP087634:4221064-4221438 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT225771 NZ_CP121354:5485726-5486004 [Mesorhizobium sp. WSM4904]
ATGTCCAAGCCAGCCAGCGACAGAACCGAACGCTTCAATATCCGCATACGGCCCGCGCAGAAGGCTATGATCGCCCGCGC
GGCGGCGCTGTCGCACAAGGACATGACCGACTTCATCCTCGACAAGGTGCTTCCTGAGGCGGAAGCCGTCATCAAGGCGA
TCGAAAAGACCGAAGTCTCGGAGCGCGACTTCAAACGCATCCTCGAACTGCTCGAGCATCCACCGAAGCCAAATGCGAAA
TTGCGGGCCGCCATTGCAGCTCTTCCCGATACGATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References