Detailed information of TA system TA225757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2458..2932
• Replicon: plasmid pDD02341-4
• Accession: NZ_CP087633
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02341

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: LMH53_RS29345
• Protein ID: WP_004197762.1
• Coordinates: 2458..2724 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: LMH53_RS29350
• Protein ID: WP_000879771.1
• Coordinates: 2708..2932 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH53_RS29330 (LMH53_29330)	1075..1401	+	327	WP_050516389.1	hypothetical protein	-
LMH53_RS29335 (LMH53_29335)	1427..1972	+	546	WP_023317533.1	hypothetical protein	-
LMH53_RS29340 (LMH53_29340)	2038..2388	+	351	WP_004197756.1	hypothetical protein	-
LMH53_RS29345 (LMH53_29345)	2458..2724	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH53_RS29350 (LMH53_29350)	2708..2932	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
LMH53_RS29355 (LMH53_29355)	3599..3811	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
LMH53_RS29360 (LMH53_29360)	3818..4327	-	510	WP_032433972.1	MbeB family mobilization protein	-
LMH53_RS29365 (LMH53_29365)	4362..4796	-	435	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
LMH53_RS29370 (LMH53_29370)	5005..5349	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
LMH53_RS29375 (LMH53_29375)	5339..5515	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T225757 WP_004197762.1 NZ_CP087633:c2724-2458 [Klebsiella pneumoniae subsp. pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T225757 NZ_CP121309:c1334083-1333676 [Ensifer adhaerens]
ATGTTGAAATACATGCTCGACACCAATATCTGCATCTACACGGTCAGGAACCGGCCGCAGCAGGTTCGTGAGGCGTTCAA
CAGGCATCACGGCCAACTGTGCATCAGCTCGGTCAGCCTGATGGAGTTGATCTGCGGCGCGGAAAGATCCGCAATGCCCG
AGCGCAACCTATCGGTCATAGAGGGGTTTGCCGCGCGTCTTGAGGTTCTCAACTACGATCAGGCGGCAGCCAGCCATACC
GGACAGTTGCGCGCCGAACTCGCCCGCAGTGGCACGCCGATAGGGCCTTACGATCAGTTGATCGCGGGTCATGCACGGTC
TCAAGGCTTGATCCTCGTGACCAACAACCGGCGGGAGTTCGATCGTGTGCCCGGCTTGCGAGTCGAGGACTGGACCGCGG
CGCCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT225757 WP_000879771.1 NZ_CP087633:c2932-2708 [Klebsiella pneumoniae subsp. pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT225757 NZ_CP121309:c1334316-1334083 [Ensifer adhaerens]
ATGTCGGAACAGGGCGCAGTTTTTCTCACCAATCGTAGTCAGGCGATCCGGCTGCCGAGGGCCGTAGCGCTTCCGGAGGA
CGTCAAACGCGTCGATATCGTGGCGGTGGGGCGAACGCGCATCATTACACCCGCAGGCGAGGCCTGGGACAGTTGGTTCG
ACGGCGAGCTCGTCACAGCCGATTTCATGGTGCAGAGAGAGCAGCCGGAGGCCCAGGAACGCGAGCCACTCTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5