Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5335586..5336211 Replicon chromosome
Accession NZ_CP087629
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02341

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LMH53_RS26270 Protein ID WP_002882817.1
Coordinates 5335586..5335969 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LMH53_RS26275 Protein ID WP_004150355.1
Coordinates 5335969..5336211 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH53_RS26255 (LMH53_26255) 5332952..5333854 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LMH53_RS26260 (LMH53_26260) 5333851..5334486 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LMH53_RS26265 (LMH53_26265) 5334483..5335412 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LMH53_RS26270 (LMH53_26270) 5335586..5335969 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH53_RS26275 (LMH53_26275) 5335969..5336211 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LMH53_RS26280 (LMH53_26280) 5336416..5337333 + 918 WP_002882812.1 alpha/beta hydrolase -
LMH53_RS26285 (LMH53_26285) 5337347..5338288 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LMH53_RS26290 (LMH53_26290) 5338333..5338770 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LMH53_RS26295 (LMH53_26295) 5338767..5339627 - 861 WP_002882807.1 virulence factor BrkB family protein -
LMH53_RS26300 (LMH53_26300) 5339621..5340220 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T225746 WP_002882817.1 NZ_CP087629:c5335969-5335586 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T225746 NZ_CP121298:1184239-1184652 [Salmonella enterica subsp. enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT225746 WP_004150355.1 NZ_CP087629:c5336211-5335969 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT225746 NZ_CP121298:1183992-1184258 [Salmonella enterica subsp. enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References