Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4750992..4751802 Replicon chromosome
Accession NZ_CP087629
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02341

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag LMH53_RS23465 Protein ID WP_002887280.1
Coordinates 4750992..4751525 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LMH53_RS23470 Protein ID WP_002887278.1
Coordinates 4751536..4751802 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH53_RS23460 (LMH53_23460) 4749823..4750944 + 1122 WP_002887282.1 cupin domain-containing protein -
LMH53_RS23465 (LMH53_23465) 4750992..4751525 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
LMH53_RS23470 (LMH53_23470) 4751536..4751802 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LMH53_RS23475 (LMH53_23475) 4751905..4753338 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
LMH53_RS23480 (LMH53_23480) 4753328..4754011 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LMH53_RS23485 (LMH53_23485) 4754183..4755568 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
LMH53_RS23490 (LMH53_23490) 4755586..4755930 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T225743 WP_002887280.1 NZ_CP087629:c4751525-4750992 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T225743 NZ_CP121298:159051-159362 [Salmonella enterica subsp. enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT225743 WP_002887278.1 NZ_CP087629:c4751802-4751536 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT225743 NZ_CP121298:159359-159805 [Salmonella enterica subsp. enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References