Detailed information of TA system TA225728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 7773..8353
• Replicon: plasmid pDD02280-3
• Accession: NZ_CP087626
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02280

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LMH50_RS29095
• Protein ID: WP_071177730.1
• Coordinates: 7773..8087 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LMH50_RS29100
• Protein ID: WP_000093040.1
• Coordinates: 8075..8353 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH50_RS29070 (LMH50_29070)	3717..5681	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
LMH50_RS29075 (LMH50_29075)	5681..6412	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
LMH50_RS29080 (LMH50_29080)	6419..6949	+	531	WP_071177729.1	hypothetical protein	-
LMH50_RS29085 (LMH50_29085)	6976..7155	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LMH50_RS29090 (LMH50_29090)	7181..7609	-	429	WP_001140599.1	hypothetical protein	-
LMH50_RS29095 (LMH50_29095)	7773..8087	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH50_RS29100 (LMH50_29100)	8075..8353	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LMH50_RS29105 (LMH50_29105)	8528..8893	-	366	WP_072354022.1	TonB family protein	-
LMH50_RS29110 (LMH50_29110)	8890..9261	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
LMH50_RS29115 (LMH50_29115)	9535..9780	-	246	WP_032440458.1	hypothetical protein	-
LMH50_RS29120 (LMH50_29120)	10244..11800	-	1557	WP_053897648.1	PAS domain-containing methyl-accepting chemotaxis protein	-
LMH50_RS29125 (LMH50_29125)	11825..12529	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LMH50_RS29130 (LMH50_29130)	12563..13087	-	525	WP_094818827.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / sul2 / tet(A) / blaLAP-2 / qnrS1	-	1..91826	91826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T225728 WP_071177730.1 NZ_CP087626:c8087-7773 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T225728 NZ_CP121296:c2291268-2290339 [Salmonella enterica subsp. enterica]
ATGGATCTGCGTGATAGTTATGCCCGACAGGTTAGGCTATTAATGGCTGCGCTGCCGCATGTGGCAAAGGAGTCTTGCTT
CGCCCTGAAAGGCGGCACGGCAATCAATTTGTTTGTACAGGATTTCCCTCGCCTGTCGGTAGATATCGATCTGGCGTATA
AGGCCTATGCGGATCGCGATACCGATCTGGCTACCATCAATGACGCCTTGATGCGTATTACTGAATCACTGAACGGCAGA
CCGGGGATCACCGCTATCCGGCAGGAGAACAAAGCGGATGAAAAGCGCATCATTGTTAACACCGCCGACGCGCAGATAAA
GATTGAAGTTAGCCCAGTCTGGCGTGGGCTGCTGTTGCCAGCAACAGAAATGCCGGTATGTGAACAGGTGGAAATGGAGT
ATGGCTTCACCACGATGAATGTCGTCTCGCAGGCGGATCTTTACGGCGGTAAAATCTGCGCCGCGCTTGACCGCCAGCAT
CCGCGCGATCTGTTTGACGTACTGAATATGCTGGAGAAACCGGGCCTGACGCGCGAGATCTTTGACGGTTTTCTTTGCTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCGCCGAACTGGGATACATCGCGGATCGCAACTCTTTACGCAC
AGGAATTTTCAGGAATGACGCAGCGGGAAACATCGCTGGAGTCACTTTTATCAGTAACGACTTTGCTGCCACAAACGCTG
AAATCGCATCTTACGGACAGAGATCGCCAGTTCTTGCTCAGCTATAAGCAAATTACCCCTGACTGGTCGCTGTATCGCTA
TCCTGAAATTCAACACCTACCGGCTATTCGCTGGAAGCTGCGTAATCTGGCGGTACTGAAGGAAAAGAACCCGGCCAAGT
ATGCGGCGACGGTGAACAAGTTAGAAGGCGTTCTGGAGCAGTACTTTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT225728 WP_000093040.1 NZ_CP087626:c8353-8075 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT225728 NZ_CP121296:c2292037-2291255 [Salmonella enterica subsp. enterica]
TTGCCAAACTATCTAAACTGGCTGATGCAAAACACCCAACCCGGTCAGGTGTTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTTTCTTACCTCTATGTCCAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTTTACTGCCGGACGG
GCCGTGAGCCAGACTGGGTGGACGCGGTTTATTGCCTGCAAACGCAGTGGGAAAAACCGGTTCGCGTGGCGGGGCTAACC
AGCCTTGCGCTACAGGGCCATGCCCACTATACCGAACCGGGTAAACCACAGGTCTGGCTCGCGCTTCCGGCTTATGTCAA
GCTGCCGGGATGGTTCGCCGCCTTTGAACAATCCGCGATGTTTGTCACGCTGTATACCTCTGGCCTCACCGTTGATGTAA
CACCATTTACGACAACACTGAATATCGGCGACCGACAGTTAACAGGCAGTGTCCCGGAACTGGCCGCTTATGAGATCGCC
GGCGGTGTTCCCTCGTTGATCTCATTCGAACATGCCGATGCGCTGTTTCAGGGGATGAACCTTCTCAGCCCGCGTAAATT
ACAGACACTGCTCTGCGCCAGCCATTCGGTTAAAACCAACCGGGTAATGCTCTATCTTGCCCGGCGCAATGAGCATCCTT
ATTTTCGGTATCTCGATCAAAGCGATATAGAAACAGGTACGGGAAAGCGGCAAATCGTTCCCGGCGGCTGGCTGGAGCCG
GATTATCAAATCACCGTTCCCCGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1