Detailed information of TA system TA022572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 3437814..3438487
• Replicon: chromosome
• Accession: NC_010125
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A9H385
• Locus tag: GDI_RS15845
• Protein ID: WP_012227838.1
• Coordinates: 3438065..3438487 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A9H383
• Locus tag: GDI_RS15840
• Protein ID: WP_012227836.1
• Coordinates: 3437814..3438068 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDI_RS15830	3436042..3436515	+	474	WP_012227833.1	transcription elongation factor GreA	-
GDI_RS15835	3436526..3437722	+	1197	WP_012227835.1	threonine ammonia-lyase	-
GDI_RS15840	3437814..3438068	+	255	WP_012227836.1	plasmid stabilization protein	Antitoxin
GDI_RS15845	3438065..3438487	+	423	WP_012227838.1	type II toxin-antitoxin system VapC family toxin	Toxin
GDI_RS15850	3438543..3438755	-	213	WP_012227840.1	dodecin family protein	-
GDI_RS15855	3438808..3439458	-	651	WP_012227842.1	uracil phosphoribosyltransferase	-
GDI_RS15860	3439455..3439838	-	384	WP_012554711.1	peptide-methionine (R)-S-oxide reductase MsrB	-
GDI_RS15865	3439941..3441491	+	1551	WP_012227846.1	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	-
GDI_RS15870	3441942..3442841	+	900	WP_012227848.1	peptidoglycan DD-metalloendopeptidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15216.50 Da
• Isoelectric Point: 4.8485

>T22572 WP_012227838.1 NC_010125:3438065-3438487 [Gluconacetobacter diazotrophicus PA1 5]
MILLDTNVVSEPWKPVPEPRVLEWIDAQAIETLWLSAVTVAELRFGIAAMPAGRRRSILHQSLEQDVLPLFDGRVLPFDL
AASQAYADLMMRARSDGRAIGQADGYIAAIAASRGYAVASRDVSPFEAAGVKILNPWQGA

Nucleotide

• Length: 423 bp

>T22572 NC_010125:3438065-3438487 [Gluconacetobacter diazotrophicus PA1 5]
ATGATCCTTCTTGATACCAATGTGGTCTCGGAGCCATGGAAGCCTGTTCCTGAACCGCGTGTCCTGGAATGGATCGACGC
CCAGGCGATCGAGACGCTGTGGCTGTCGGCCGTCACGGTGGCGGAATTGCGATTCGGAATTGCCGCCATGCCGGCAGGCC
GGCGGCGTTCGATCCTGCATCAGAGTCTGGAACAGGACGTTCTGCCCCTGTTCGACGGGCGTGTCCTGCCCTTCGATCTG
GCGGCGTCCCAGGCTTATGCAGATCTGATGATGCGGGCCAGGTCCGATGGACGGGCGATCGGCCAGGCGGACGGCTATAT
TGCCGCAATTGCCGCATCCCGCGGTTACGCGGTGGCCAGTCGGGATGTGTCGCCGTTCGAGGCGGCCGGTGTGAAGATCC
TGAATCCGTGGCAGGGCGCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9236.54 Da
• Isoelectric Point: 6.7262

>AT22572 WP_012227836.1 NC_010125:3437814-3438068 [Gluconacetobacter diazotrophicus PA1 5]
MSSVVIRNLPEETHRALKARAALHGHSTEAEIRAILEAVVRPPKRVRLGSLLASIGREADVTEQDVAALQQVRDQTPAEP
MTFE

Nucleotide

• Length: 255 bp

>AT22572 NC_010125:3437814-3438068 [Gluconacetobacter diazotrophicus PA1 5]
ATGTCCTCTGTCGTCATCCGCAATCTTCCCGAAGAGACGCATCGGGCGCTGAAAGCGCGGGCCGCCCTGCATGGACACAG
CACGGAAGCGGAAATCCGCGCCATTCTGGAAGCCGTCGTCCGGCCGCCGAAGCGCGTCAGGCTGGGGTCGCTTCTGGCGT
CCATAGGTCGTGAAGCCGACGTGACCGAGCAGGATGTCGCGGCCTTGCAGCAGGTTCGGGATCAGACCCCAGCCGAGCCG
ATGACATTCGAATGA

Structures

Toxin

• AlphaFold DB: A9H385

Antitoxin

• AlphaFold DB: A9H383